You are here

NEW METHODS FOR SYNTHESIS OF FLUORESCENT DNA

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7296
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1987
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
10457 Roselle Street Suite E
San Diego, CA 92121
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 RICHARD H TULLIS PHD
 (619) 587-0320
Business Contact
Phone: () -
Research Institution
N/A
Abstract

THE GOAL OF THIS RESEARCH IS TO DEVELOP REAGENTS AND METHODSFOR THE DIRECT CHEMICAL SYNTHESIS OF FLUORESCENT DNAS DESTINED FOR USE AS DNA HYBRIDIZATION PROBES AND IN PRIMER-DIRECTED DNA SEQUENCING. RECENTLY, HOOD AND COWORKERS HAVE DEVELOPED A PRIMER-DIRECTED DNA SEQUENCING TECHNIQUE. DNA PROBES HAVE ALSO BEEN FOUND TO BE USEFUL FORDETECTING BACTERIA AND VIRUSES AS WELL AS GENETIC ABNORMALITIES. SEVERAL COMPANIES ARE CURRENTLY DEVELOPING DIRECT FLUORESCENT PROBES FOR USE IN SUCH APPLICATIONS WITH THE ULTIMATE GOAL OF PRODUCING RAPID, SENSITIVE, AND RELIABLE CLINICAL TESTS. CURRENTLY, MOST SYNTHETIC PROCEDURES FOR MAKING FLUORESCENT DNA PROBES RELY ON SECONDARY ENZYMATIC AND CHEMICAL MODIFICATION. THESE DEVELOPMENTS WOULD BE SPEEDED BY THE INTRODUCTION OF METHODSFOR DIRECT SYNTHESIS OF THE DESIRED PRODUCT. IN PHASE I, NOVEL FLUORESCENT-LABELED NUCLEO-TIDE PHOSPHORAMIDITIES WILL BE SYNTHESIZED THAT ARE SUITABLE FOR USE IN AUTOMATED DNA SYNTHESIZERS. THESE COMPOUNDS WILL BE USED TO SYNTHESIZE THE FLUORESCENT OLIGONUCLEOTIDES. THE GENERAL AVAILABILITY OF SUCH COMPOUNDS SHOULD SIGNIFICANTLY STIMULATE PROGRESS IN THE USE OF FLUORESCENT PROBES AS NONISOTOPIC DIAGNOSTIC AND SEQUENCING REAGENTS.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government