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NOVEL ENZYME IMMOBILIZATION FOR BIOSENSOR APPLICATIONS (BACTERIA)

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16640
Amount: $49,825.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1991
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
40 Allston Street
Cambridge, MA 02139
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Navia, Manuel A
 Principal Investigator
 (617) 576-3111
Business Contact
 1 R43 GM46164- 01
Phone: () -
Research Institution
N/A
Abstract

ENZYME CRYSTALS, CROSS-LINKED USING A BIFUNCTIONAL REAGENT SUCH AS GLUTARALDEHYDE, CONSTITUTE A NOVEL FORM OF ENZYME IMMOBILIZATION. SUCH CROSS-LINKED IMMOBILIZED ENZYME CRYSTALS (CLIECS) ARE SUPERIOR TO CONVEN- TIONALLY IMMOBILIZED ENZYMES IN MANY RESPECTS. FOR EXAMPLE, THE CRYSTAL LATTICE INTERACTIONS, SUPPLEMENTED BY CHEMICAL CROSS-LINKS, PROVIDE THE INDIVIDUAL ENZYME MOLECULES WITHIN A CLIEC WITH AN ENHANCED STABILITY THAT ALLOWS FOR FUNCTION IN HARSH ENVIRONMENTS (SUCH AS ELEVATED TEMPERATURES AND UNUSUAL SOLVENTS) THAT WOULD NOT OTHERWISE BE COMPATIBLE WITH CATALYTIC ACTIVITY. THESE FEATURES OF CLIECS LEND THEMSELVES TO BIOSENSOR APPLICATIONS, WHERE IT IS DESIRABLE TO PRODUCE THE LARGEST POSSIBLE SIGNAL FROM THE SMALLEST POSSIBLE VOLUME OF CATALYST AND QUANTITY OF SUBSTRATE. IN THE PHASE I STUDY, CLIECS OF BACTERIAL LUCIFERASE FROM "VIBRIO HARVEYI" WILL BE INVESTIGATED IN A PROTOTYPE SYSTEM TO DEMONSTRATE THE FEASIBILITY OF THIS APPROACH TO BIOSENSOR APPLICATIONS. LUCIFERASE IS CHOSEN BECAUSE IT PRODUCES LIGHT AS CONSEQUENCE OF ITS CATALYTIC ACTIVITY. THE EXCEPTIONAL SENSITIVITY AND EFFICIENCY OF PHOTON SENSING DEVICES CAN BE EXPLOITED TO DETECT FEMTOMOLAR CONCENTRATIONS OF SELECTED ANALYTES UNDER APPROPRIATE EXPERIMENTAL CONDITIONS. A BROAD ARRAY OF CLINICALLY INTERESTING ENZYMATIC REACTIONS HAVE BEEN DESCRIBED IN THE LITERATURE THAT CAN BE READILY COUPLED TO LIGHT PRODUCTION BY A LUCIFERASE CLIEC.

* Information listed above is at the time of submission. *

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