You are here

High throughput Methods for Sequencing and Analysis

Description:

With the advent of faster and cheaper genomic sequencing technologies, the microbial, plant and metagenomics communities will see an order of magnitude increase in sequencing DNA and RNA to infer microbial, plant and community information. Applications are sought in the following areas:

  • High throughput experimental methods for functional assignment/annotation of genes and
  • genomes of relevance to the DOE mission.
  • High throughput automated or robotic sample preparation methods focused on DNA and/or
  • messenger RNA.
  • Novel DNA sequencing technologies that generate at least 2 Kbases of continuous sequence,
  • with base call error rates less than 5%
  • New methods for isolation of DNA or RNA from single cells with accurate amplification and
  • sequencing of >90% of the genomic content.
  • New methods for direct sequencing of up to 400 base pairs of RNA, not requiring any
  • intermediate cDNA steps
  • New software tools for high throughput and efficient assembly of short read (150< base
  • pairs) fragments of DNA from disparate sequencing platforms.
  • New methods for accurate separation of distinct genomes from polyploid (multiplied) nuclei
  • of fungi and plants.

Questions – contact John Houghton, john.houghton@science.doe.gov

US Flag An Official Website of the United States Government