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Microfluidic High-throughput Platform for Determining Kinetic Constants of Enzyme Variants

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W81XWH-10-C-0211
Agency Tracking Number: C2-0336
Amount: $749,815.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: CBD10-107
Solicitation Number: 2010.1
Solicitation Year: 2010
Award Year: 2011
Award Start Date (Proposal Award Date): 2011-04-05
Award End Date (Contract End Date): N/A
Small Business Information
215 Wynn Dr., 5th Floor
Huntsville, AL -
United States
DUNS: 185169620
HUBZone Owned: No
Woman Owned: Yes
Socially and Economically Disadvantaged: No
Principal Investigator
 Ketan Bhatt
 Senior Research Engineer
 (256) 327-0666
Business Contact
 Deborah Phipps
Title: Senior Contracts Speciali
Phone: (256) 726-4884
Research Institution

Current high-throughput platforms rely on labeled substrates that produce detectable spectroscopic signals upon reaction for monitoring enzyme catalytic activity. Add to that the high price tag for these instruments, ranging from several hundred thousand to millions of dollars, and there is a clear need for alternate solutions for a label-free, low-cost, high-throughput enzyme screening platform. We propose to develop a novel, microfluidic, high-throughput platform for determining enzyme kinetics. Specifially, the platform will utilize impedance measurements for identifying enzyme variants with improved affinity for organophosphorus (OP) agents. The platform features multiple parallel processors that generate a gradient of reagent concentrations while seamlessly integrating sample metering, mixing and incubation functionalities using on-chip pneumatic valving. Proof-of-concept was provided during Phase I, by monitoring the hydrolysis of OP agent simulants using organophosphorus hydorlase. During Phase II, we will refine and optimize the platform using multi-physics based simulations. Prototypes will be fabricated using state-of-art microfabrication techniques and detailed experimental characterization would be carried out to demonstrate platform operation. We have assembled an interdisciplinary team of engineers and scientists from CFDRC and Auburn University with expertise in design, modeling, fabrication and experimental characterization of microfluidic systems for a successful development of the proposed platform.

* Information listed above is at the time of submission. *

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