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Development of a universal method for diagnostic sample inactivation, extraction and enrichment of pathogens in arthropod hosts

Award Information
Agency: Department of Defense
Branch: Defense Health Agency
Contract: W81XWH-10-C-0175
Agency Tracking Number: O2-1059
Amount: $749,988.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: OSD09-H19
Solicitation Number: 2009.3
Solicitation Year: 2009
Award Year: 2011
Award Start Date (Proposal Award Date): 2011-09-12
Award End Date (Contract End Date): N/A
Small Business Information
14 Norfolk Ave
South Easton, MA -
United States
DUNS: 125354308
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Alexander Lazarev
 Vice President of R&D
 (508) 230-1828
Business Contact
 Richard Schumacher
Title: President and CEO
Phone: (508) 230-1828
Research Institution

We propose to develop an easy-to-use, safe, automated, field-portable high-throughput system, with related methods and consumables, to allow for the simultaneous inactivation and extraction of target molecules from pathogenic organisms, such as viruses, and rickettsia and non-rickettsial bacteria, that propagate and/or disseminate in arthropod hosts (herein referred to as Agents). The proposed System will use a combination of mechanical homogenization, high hydrostatic pressure, elevated temperature, and optimized chemical reagents to inactivate said Agents and extract the target molecules (DNA, RNA and proteins) from host organisms for subsequent separation and detection by a variety of conventional methods. We propose that the System would be equally effective in the field and in a laboratory environment. All sample processing will occur in sealed sample containers. These containers will facilitate sample homogenization, lysis, and rapid extraction of target molecules. Additionally, they will be suitable for sample collection, transportation, and storage (pre- and post analysis). Such containers will help ensure strict chain-of-custody, prevent sample cross-contamination, and reduce the likelihood of exposing personnel to infectious Agents. The System will be made rugged and portable; will be capable of automating the steps of sample inactivation, fractionation, enrichment, and extraction; and will be deployable in the field environment.

* Information listed above is at the time of submission. *

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