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Yeast Artificial Positive Feedback Loop as Tool to Enhance Multigenes Metabolic Pathways


The JBEI has developed a technology that employs in yeast cells a trait-changing strategy that has been applied to fine tune desirable biomass deposition in plants. Crucial to this strategy is the design of a genetic switch, or transcription factor, containing an artificial positive feedback loop (APFL) within its DNA sequence. Once inserted in yeast, the switch regulates expression of desired new traits, while the embedded feedback loop induces increased transcription of the switch itself, sustaining the production of those traits. The APFL strategy was first employed successfully to fine tune secondary cell wall synthesis in the model plant Arabidopsis. The new JBEI technology extends this strategy with a feedback loop that works in yeast, which is a model organism for many types of fungal cells. By identifying key genes in yeast that can be controlled in this manner, the researchers have demonstrated that this technology for plants can be adapted to entirely different organisms. In yeast, it confers traits that can potentially transform fungal cell cultures into efficient factories for the production of chemicals ranging from biofuels to pharmaceuticals.
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