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Production of 1-Deoxyxylulose-5-Phosphate Via Enzymatic Dehydration-Reduction of Xylose-Derived Sugar


Researchers at the JBEI have generated a new synthetic pathway in cells to 1-deoxyxylulose-5-phosphate (DXP). Both routes allow more direct conversion of carbon to terpenoid compounds circumventing the typical, but inherently inefficient, route to DXP. The JBEI process results in the conservation of 17% of carbon being converted to terpenoid products. The novel pathways to DXP entail conversion of xylulose-5-phosphate to DXP, which circumvents the loss of CO2 and provides a higher theoretical yield, particularly if xylose is included as a carbon source. It also provides a second metabolite pool (the essential pentose phosphate pathway) for isoprenoid biosynthesis. In the case of having a mixed carbon source (for example, xylose and glucose from a hemicellulose feedstock), it is envisioned that a large fraction of the xylose component could be primarily converted to the isoprenoid product since the carbon is diverted at the entry point into metabolism (xylulose-5-P). The novel routes into the DXP pathway could also be used in conjunction with the normal DXP-mediated route to maximize flux.
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