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Translation-Coupling Cassette for Quickly and Reliably Monitoring Protein Translation in Host Cells


UWMadison GLBRC researchers have developed a method of using translation coupling to quickly and reliably determine whether a given host is capable of expressing the gene product of any given gene. This method could be used to monitor protein translation efficiency in bacterial cells which can be very important in the discovery and screening work around producing microbes to ferment biomass-derived sugars to biofuels and biorenewable chemicals. This method utilizes antibiotic resistant in a way that confers resistance only if the transgene is translated into protein, allowing for more real time monitoring of recombinant protein production.
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