DIPAIN based assay for the T-2 Toxin

This proposed project is to develop a rapid assay for T-2 Toxin. Under this project we will develop DIPAIN-derivative based test-strips that indicate the presence of trace quantities of trichothecene mycotoxins in aqueous solutions. The T-2 toxin will be used as a test case for this effort. We will use of 2-(diphenylacetyl)-l,3-indanedione-l-hydrazone (DIPAIN II) and its derivatives as reagents on solid supports, using the property that DIPAIN derivatives undergo significant fluorescence enhancement in the visible range under UV excitation when in contact with trichothecene mycotoxins and that the presence of the toxin is indicated by the enhanced fluorescence of the DIPAIN derivative.