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Quantitative dual-mode scanner for breast cancer therapy

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43GM105188-01A1
Agency Tracking Number: R43GM105188
Amount: $143,253.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIGMS
Solicitation Number: PA12-088
Timeline
Solicitation Year: 2013
Award Year: 2013
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
11011 Torreyana Road
SAN DIEGO, CA 92121-
United States
DUNS: 9495107
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 ALAN GREENER
 (858) 457-1919
 agreener@genetherapysystems.com
Business Contact
 ANTHONY SORGE
Phone: (858) 457-1919
Email: asorge@genetherapysystems.com
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): Efficient delivery of DNA into E. coli remains a major limitation in the construction of high complex and representative gene libraries. The most efficient way developed to date is using electroporation - delivering anelectric discharge to cell creating channels through which DNA can enter. We have created a mutant E. coli strain through a novel evolution process that results in a significantly more robust strain capable of surviving the electroporation process, and itconcomitantly exhibits an improvement in efficiency. However, the efficiency with ligated DNA - the substrate that must be introduced into E. coli during library construction - remains highly inefficient. We propose to extend this evolutionary process to create variants that are better suited to accept larger, ligated DNA molecules. Understanding the molecular basis of these mutants will provide a template for how to create improved hosts of other bacterial species. In addition, identification of the mutation(s) responsible for resistance to electric shock it may provide insights into survival pathways in bacteria and higher organisms. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: Preparing large and representative gene libraries is an important component for both basic science and in the evolution of proteins as therapeutics and diagnostics. A major limitation to the construction of these libraries is the ability to introduce he material efficiently into E. coli. We propose to create an E. coli host to overcome this limitation

* Information listed above is at the time of submission. *

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