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FY 2013 Contract HHSN271201300011C SBIR-I, Topic 004, RASL-Seq HTS Tox Profiler

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: N43DA130011
Agency Tracking Number: N43DA130011
Amount: $149,658.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIDA
Solicitation Number: N/A
Timeline
Solicitation Year: 2013
Award Year: 2013
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
5758 SAN ELIJO
RANCHO SANTA FE, CA 92067-0402
United States
DUNS: 78410758
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOANNE YEAKLEY
 (760) 525-1825
 JOELMCCOMB@YAHOO.COM
Business Contact
 JOANNE YEAKLEY
Phone: (760) 525-1825
Email: JOELMCCOMB@YAHOO.COM
Research Institution
 Stub
Abstract

The Contractor shall develop and implement a highly multiplexed targeted sequencing assay designed to detect toxicity during compound screening. The RASL-Seq HTS assay will monitor gene expression across 184 target genes and 20 housekeepi ng genes, in a format amenable to high throughput screening (HTS) that is cost effective and easy to use. Phase I will characterize assay performance using a set of 10 reference compounds and selected compounds from the National Toxicology Program (NTP), using HepG2 cellsin a 384- well plate context. We will demonstrate assay utility using an HTS protocol that can test up to 10,000 compound treatments per day, while providing quantitative, precision dose response data. In Phase II, we will expand this assay to the RASL-SeqHTS Tox Profiler assay, which will include the -1100 toxicity-related genes exhibiting known expression changes, and a surrogate whole genome pathway and mechanism assay of 1,000 to 3,000 genes. We will establish whether a 1536-well format can be used, and will design an automated process that can test 100,000 compounds per day. PUBLIC HEALTH RELEVANCE

* Information listed above is at the time of submission. *

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