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FY 2013 Contract HHSN271201300012C, SBIR-1, Topic 003, Automated Instrument for C

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: N43DA130012
Agency Tracking Number: N43DA130012
Amount: $158,510.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIDA
Solicitation Number: N/A
Solicitation Year: 2013
Award Year: 2013
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
CODEX BIOSOLUTIONS, INC. 401 Professional Drive, Suite 160
Gaithersburg, MD 20879-
United States
DUNS: 963268094
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: Yes
Principal Investigator
 (301) 538-8802
Business Contact
Phone: (301) 538-8802
Research Institution

In this SBIR phase I grant application, Codex BioSolutions proposes to construct a cell-based high throughput assay that measures chemical toxicity using a novel dye, m-MPI. This dye was engineered to overcome many of the drawbacks of current dyes used tomeasure mitochondrial membrane potential. The parent dye JC-1 has been used for this purpose previously. In the mitochondria, where it accumulates as aggregates, it fluoresces red, whereas in the cytoplasm where it is a monomer, it fluoresces green. However, JC-1 is difficult to use because it has very low water solubility which makes using its differential fluorescent properties difficult. Our dye, m-MPI has increased solubility allowing easy quantitative assessment of MMP status simply by calculating theratio of red/green fluorescence. m-MPI can also be used with cells that do not work with JC-1 (such as primary cells) due at least in part to its increased sensitivity. We believe our assay will more accurately assess hazards in a shorter time and with less cost. PUBLIC HEALTH RELEVANCE

* Information listed above is at the time of submission. *

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