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Nanobodies for detecting and manipulating A to I editing enzymes and their modified RNA products

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43DA038980-01
Agency Tracking Number: R43DA038980
Amount: $224,975.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIDA
Solicitation Number: DA15-001
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
271 GREAT VALLEY PARKWAY SUITE 300
MALVERN, PA 19355-1326
United States
DUNS: 962964990
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 HIEP TRAN
 (610) 990-7531
 tran@abzymetx.com
Business Contact
 HIEP TRAN
Phone: (610) 990-7531
Email: tran@abzymetx.com
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): Adenosine to inosine or A-to-I RNA editing executed by ADAR family proteins is abundant in the central nervous system (CNS). The availability of tools to monitor and manipulate both ADAR enzymes and A-to-I RNA modifications will facilitate investigation of the relationships between specific A-to-I conversions and their function in CNS development. The goal of this project is to develop a set of nanobodies to A-to-I RNA editing enzymes and their modified RNA products. These nanobodies are expected to serve not only as reagents for detection, quantification, or immunoprecipitation but also as intracellular antibodies capable of inhibiting the activity of target ADAR enzymes or binding to specific A-to-I modified RNAs in cells. As proof-of-principle, in Phase I, using Abzyme's proprietary self-diversifying single domain antibody library platform for high throughput antibody discovery, nanobodies to inosine-containing generic RNAs and site- specific A-to-I mRNA isoforms

* Information listed above is at the time of submission. *

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