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RASL-Seq for Preserved Tissues in Toxicity Testing

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43ES024107-01
Agency Tracking Number: R43ES024107
Amount: $149,871.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIEHS
Solicitation Number: ES13-009
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
5758 SAN ELIJO
RANCHO SANTA FE, CA 92067-0402
United States
DUNS: 78410758
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOANNE YEAKLEY
 (760) 525-1825
 joyeakley@gmail.com
Business Contact
 JOANNE YEAKLEY
Phone: (760) 525-1825
Email: joyeakley@gmail.com
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): This project will develop and characterize the application of RASL-Seq, a multiplexed targeted expression profiling technology that uses q sequencing read-out, to analysis of banked tissues. The technology detects hundreds of RNA targets at once in cell lysates, using oligo ligation, amplification, and next-generation sequencing for quantitation. This highly multiplexed assay will allow a simple, fixed tissue assay protocol that can be applied to different rodent tissues, while delivering a simple expression profiling report. The technology will promote toxicology research by enabling direct comparisons between expression profiles derived from compound screening in vitro to those observed in animals dosed with the same compounds. It will do so at a low cost/sample by multiplexing 20 to 100 or more samples/sequencing run, thus reducing the sequencing cost/sample to 6 from 600. We will optimize the lysis buffer/protocol for formalin-fixed paraffin-embedded (FFPE) tiss

* Information listed above is at the time of submission. *

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