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Detachable Nozzle Piezoelectric Dispense System for Cryo-TEM Sample Preparation

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43GM108126-01
Agency Tracking Number: R43GM108126
Amount: $149,800.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIGMS
Solicitation Number: PA11-215
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
PHOENIX, AZ 85048-8972
United States
DUNS: 828898879
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (602) 206-9933
Business Contact
Phone: (602) 206-9933
Research Institution

DESCRIPTION (provided by applicant): Project Summary / Abstract Engineering Arts (EA) proposes the development of a detachable nozzle piezoelectric dispense system for use in Cryo-TEM (Cold - Transmission Electron Microscopy) sample preparation instrumentation. Cryo-TEM is an important tool for studying the structure of biological macromolecules in their native aqueous state. The challenging and laborious process of preparing 'thin-ice' frozen aqueous samples for Cryo-TEM has remained essentially unchangedfor decades. Typically a single aqueous biological sample (~3 l) is manually applied onto a thin porous Electron Microscopy (EM) grid which is then blotted to remove excess sample and then plunged into liquid-ethane for flash freezing. This process hopefully leaves enough regions on the grid with the proper vitrified 'thin-ice', typically 50 to 200nm thickness, required for EM imaging. The detachable piezoelectric nozzle dispense system proposed here is a key innovation that will lead to productivity,

* Information listed above is at the time of submission. *

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