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Automated system for unbiased and high yield preparation and quantitation of genomic samples

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43HG007620-01
Agency Tracking Number: R43HG007620
Amount: $150,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NHGRI
Solicitation Number: PA13-088
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
5147 TRINITY PARK DR
ALVISO, CA 95002-0081
United States
DUNS: 78771591
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 AMY HIDDESSEN
 (650) 248-9886
 amy@purigenbio.com
Business Contact
 KLINT ROSE
Phone: (650) 248-9886
Email: klint@purigenbio.com
Research Institution
 Stub
Abstract

Summary Sample preparation of nucleic acids is essential for all genomic analyses, and yet it is the primary source of variability and requires tedious manual labor. For samples with relatively low abundance (lt100 ng or roughly 20,000 mammalian cells or less), current sample preparation methods also result in crippling material loss and low repeatability. For small RNA, current technologies can result in severe sequence or length biases. We here propose a novel method and product which achieves nucleic acid extraction, purification, and quantitation from complex samples using isotachophoresis (ITP). ITP is an electrophoresis method which can be used to highly selectively extract target nucleic acids from complex samples including blood, urine, and cell culture. Proposed is the development of novel, low-cost plastic chips and a benchtop reader and control unit for automated extraction of DNA or RNA from complex lysate samples. The method offers the unique capability of processing low abundance samples: Provid

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