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Ultrahigh-throughput single-cell genetic analysis and sorting with PACS

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43HG007814-01
Agency Tracking Number: R43HG007814
Amount: $222,889.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NHGRI
Solicitation Number: PA11-335
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
953 Indiana St.
San Francisco, CA 94107-3007
United States
DUNS: 968684261
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 DENNIS EASTBURN
 (415) 513-7728
 eastburn@missionbiology.com
Business Contact
 DOUGLAS CRAWFORD
Phone: (510) 759-6972
Email: silver@missionbiology.com
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): The goal of Torrent Bio, Inc. is to commercialize a technology capable of ultrahigh-throughput screening of single-cells using PCR. The system is in many ways analogous to Fluorescent Activated Cell Sorting (FACS), except that rather than sorting intact cells, it isolates individual cells into individual aqueous picoliter-volume drops suspended in oil and performs PCR reactions on their lysates. Using multiplexed TaqMan PCR assays, the system is able to interrogate eachcell for the expression of specific combinations of genes, mutations or non-coding RNAs. Moreover, similar to FACS, this system can sort the cell lysate into different containers, allowing the lysates of the cells with a unique transcriptional fingerprintto be recovered for additional analysis using microarrays, Next Gen Sequencing, or other methods. The principal advantage of this system is that it combines the high-throughput single-cell screening and sorting capacity of FACS with the sensitivity of

* Information listed above is at the time of submission. *

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