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Digital PCR in a microplate format: high-throughput, precise DNA quantification

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43HG008121-01
Agency Tracking Number: R43HG008121
Amount: $156,080.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NHGRI
Solicitation Number: PA11-335
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
1254 MAIN ST
WALTHAM, MA 02451-1739
United States
DUNS: 168313364
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (617) 901-1830
Business Contact
Phone: (857) 284-2562
Research Institution

DESCRIPTION (provided by applicant): Quantitative PCR (qPCR) is the current industry standard for quantification of DNA samples, and is used commonly in diagnostics and research. Digital PCR (dPCR) presents numerous advantages over qPCR such as increased precision and sensitivity and absolute quantification without comparison to standards. However, current digital PCR systems require complex workflows and are not easily scaleable. We have produced a prototype of an injection molded 96 sample microplate fordigital PCR. Injection molding allows the production of the microplates with excellent feature reproduction and low costs per sample. Preliminary testing has shown the microplates to be capable of outperforming qPCR in several limited applications such aslow copy number variation determination or low resolution sample quantitation. All 96 samples can be partitioned and thermocycled at once, which results in much higher throughput than any current dPCR system. Our system also features compatibility with liq

* Information listed above is at the time of submission. *

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