Identification and Quantification of Sequence Specific RNA Modifications via the Alpha-Hemolysin Latch Zone

DESCRIPTION (provided by applicant): During this STTR program, Electronic BioSciences (EBS) proposes to develop a nanopore based system around our University of Utah collaborators newly developed technology that will be capable of identifying and quantifying sequence specific RNA modifications. A system capable of quantitative, low cost, high throughput, RNA modification characterization has the capability to elucidate the roles of these modifications in cellular signaling, and improve our understanding ofgene regulation and associated disease states. The goal of this Phase I program is to demonstrate proof-of-concept for the RNA modification characterization concept, and determine the parameters that will allow our team to develop a complete RNA modification characterization system during a follow-on Phase II program. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: This program is aimed at developing a new method and system for the characterization of sequence specific RNA modifications. This system will