Award Data

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ONE OF THE MAJOR CHALLENGES IN THE PRODUCTION OF THERAPEUTICALLY USEFUL PROTEINS, INCLUDING PRODUCTS OF RECOMBINANT DNA TECHNOLOGY, IS RAPID AND COST-EFFECTIVE PURIFICATION OF THE PROTEINS. HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY IS A POWERFUL NEW TECHNIQUE FOR PROTEIN PURIFICATION AT PRODUCTION SCALE. THE USE OF THIS TECHNIQUE HAS BEEN HAMPERED BY THE EXPENSE AND DIFFICULTY OF CUSTOM COUPL ...

THE OVERALL GOAL OF THIS PROJECT IS TO DEVELOP IMMUNOASSAYS CAPABLE OF DETECTING DIDEMNINS AT THE NANOGRAM LEVEL. DIDEMNIN B IS THE MOST POTENT ANTITUMOR, ANTIVIRAL, AND CYTOTOXIC MEMBER OF A FAMILY OF FIVE CYCLIC DEPSIPEPTIDES ISOLATED FROM MARINE TUNICATES. THIS COMPOUND IS CURRENTLY IN CLINICAL TRIALS AS AN ANTITUMOR AGENT. AVAILABLE BIOASSAY AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHODS F ...

IMMUNOCHEMICAL TECHNIQUES HAVE MANY DIVERSE APPLICATIONS. HOWEVER, THEY ARE RESTRICTED TO USE ONLY IN AQUEOUS MEDIA. ECONOMICAL TECHNIQUES FOR DIRECT ORGANIC PHASE ANALYSIS AND IMMUNOAFFINITY ISOLATION WOULD SIGNIFICANTLY BROADEN THE USEFULNESS OF ANTIBODY-BASED TECHNOLOGY. THE PURPOSE OF THIS PROJECT IS TO DETERMINE THE FEASIBILITY OF EXPLOITING ANTIGEN-ANTIBODY BINDING IN THE ORGANIC PHASE. A SE ...

THE PROPOSED RESEARCH WILL INVESTIGATE THE ABILITY OF NEUROSPORA CRASSA TO EXPRESS AND SECRETE BIOLOGICALLY-ACTIVEMAMMALIAN PROTEINS. THE ABILITY TO GENETICALLY ENGINEER BACTERIA AND YEASTS FOR THESE TASKS IS WELL DOCUMENTED. HOWEVER, RECOMBINANT PRODUCTS FROM THESE ORGANISMS OFTEN REQUIRE COSTLY DOWNSTREAM PURIFICATION AND PROCESSING. THE PHYSIOLOGY OF THE FILAMENTOUS FUNGI INDICATE THAT THEY ARE ...

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ENZYMES OF INTEREST IN CHEMICAL/BIOLOGICAL DEFENSE, PARTICULALY OXIDASES AND PEROXIDASES, WOULD BE MORE USEFUL IF THEY COULD WITHSTAND HIGH TEMPERATURES AND HAD A LONGER SHELF LIFE. THERMALLY LABILE FORMS NOW AVAILABLE COULD BE SYNTHETICALLY STABILIZED BUT THIS WOULD BE EXPENSIVE. MICROORGANISMS EXISTING IN HIGH TEMPERATURE HABITATS SURVIVE SUCH CONDITIONS BECAUSE THEY PRODUCE HEAT STABLE ENZYMES. ...

THE U.S. POSSESSES HUGE COAL RESERVES, BUT SULFUR DIOXIDE (SO2) RELEASED UPON COMBUSTION IS A MAJOR CONTRIBUTOR TO ACID RAIN. SIGNIFICANT ADVANCES HAVE BEEN MADE IN THE DEVELOPMENT OF MICROBIAL PROCESSES FOR PRECOMBUSTION REMOVALOF INORGANIC SULFUR FROM COAL. THIOBACILLUS AND SULFOLOBUS SP. HAVE BEEN MOST FREQUENTLY EMPLOYED. THEY ARE OPTIMALLY ADAPTED TO FUNCTION AT A PH BELOW 3 AND OFTEN AT ELEV ...

THIS PROPOSAL DESCRIBES A UNIQUE AND HIGHLY SIMPLIFIED IMMUNOLOGICAL PROCEDURE USING A MONOCLONAL ANTIBODY TO CIGUATOXIN IN A TWO-STEP PROCEDURE. THE MONOCLONAL ANTIBODY(MAB) IS PREPARED BY THE MOUSE HYBRIDOMA TECHNIQUE, USING HIGHLY PURIFIED CIGUATOXIN CONJUGATED TO PROTEIN CARRIERS. THE MAB-CTX THUS OBTAINED IS CONJUGATED TO COLLOIDAL GOLD (< 0.45 UM DIAMETER) OR COLORED LATEX PARTICLES. THIS WI ...

THE PROPOSED ULTRASONIC SPATIAL SENSING AID FOR THE BLIND IS AN ELECTRONIC TRAVEL AID DESIGNED TO PROVIDE THE BLIND TRAVELER WITH SPATIAL CONTEXTUAL INFORMATION, A NECESSARY PREREQUISITE FOR MOBILITY. THE DEVICE OPERATES ON THE ECHOLOCATING PRINCIPLE, BY BROADCASTING ULTRASOUND WHICH IS REFLECTED BY ENVIRONMENTAL OBJECTS. THE RETURNING ULTRASONIC ECHOES WILL BE ELECTROACOUSTICALLY TRANSFORMED INTO ...