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PALYTOXIN IS AN EXTREMELY CYTOTOXIC LOW MOLECULAR WEIGHT NATURAL PRODUCT ISOLATED FROM SOFT CORALS OF THE GENUS, PALYTHOA. N-ACYLATED DERIVATIVES OF PALYTOXIN HAVE BEEN REPORTED TO BE UP TO 100-FOLD LESS CYTOTOXIC THAN THE UNMODIFIED TOXIN. PALYTOXIN N-ACYL DERIVATIVES WHICH CAN BE CONVERTED TO THE NATIVE TOXIN BY ANTITUMOR MONOCLONAL ANTIBODY- ENZYME CONJUGATES WILL BE PREPARED AND TESTED FOR ANT ...

UREASE OFFERS DISTINCT ADVANTAGES OVER OTHER ENZYMES FOR PREPARATION OF CONJUGATES USED IN ENZYME-LINKED IMMUNO-SORBENT ASSAY SYSTEMS. MOLECULAR DEVICES CORPORATION (MENLO PARK, CA) HAS CAPITALIZED ON THESE ADVANTAGES IN THEIR THRESHOLD SYSTEM. THIS IMPRESSIVE SYSTEM UTILIZES AVIDIN-BIOTIN TO "CAPTURE" ANALYTE-BOUND ANTIBODY-UREASE CONJUGATE COMPLEXES ONTO A MEMBRANE, WHERE THEY ARE QUANTITATED BY ...

PRODUCTION OF RECOMBINANT PRODUCTS OF PHARMACEUTICAL AND INDUSTRIAL IMPORTANCE IN A VARIETY OF MICROORGANISMS HAS YIELDED A WIDE VARIETY OF BIOLOGICALLY ACTIVE PROTEINS. FUNGI OFFER THE ADVANTAGE OF REDUCED CULTURING AND DOWNSTREAM PROCESSING COSTS BECAUSE THEY ARE CAPABLE OF SECRETING PRODIGIOUS QUANTITIES OF PROTEIN. THE GENETICS AND BIOCHEMISTRY OF NEUROSPORA CRASSA ARE THE MOST FULLY CHARACTER ...

DENGUE VIRUSES ARE A LEADING CAUSE OF MORBIDITY THROUGHOUT THE TROPICS AND SUBTROPICS. ATTEMPTS TO DEVELOP LIVE ATTENUATED DENGUE VACCINES ANALOGOUS TO THE YELLOW FEVER VACINE HAVE BEEN UNSUCCESSFUL. IN AN EFFORT TO CONTRUBITE TO THE DEVELOPMENT OF A RECOMBINANT SUBUNIT DENGUE VACCINE, WE WILL INVESTIGATE EXPRESSION OF THE DEN-3 ENVELOPE GLYCOPROTEIN IN THE FILAMENTOUS FUNGUS NEUROSPORA CRASSA.

TRANSITION STATE ANALOG HAPTENS WILL BE PREPARED FOR THE ELICITATION OF ANTIBODIES WHICH CAN CATALYZE THE CONVERSION OF PALYTOXIN PRODRUGS TO PALYTOXIN.

WE WILL CONSTRUCT HYBRID POLIO/DENGUE VIRUS GENOMES IN ORDER TO DEVELOP A VACCINE FOR DENGUE VIRUS. IN THE CHIMERIC GENOMES, ONE OF TWO DENGUE VIRUS ENVELOPE GENE FRAGMENTS WILL REPLACE SEGMENT OF THE POLIO VIRUS STRUCTURAL PROTEIN GENES. SUCH CHIMERAE WOULD HAVE THE ABILITY TO REPLICATE IN THE GUT AND TO PRODUCE LARGE QUANTITIES OF IMMUNOGEN WITHOUT INFECTIOUS PARTICLE FORMATION, UNLIKE ATTENUATE ...

WE WILL ACHIEVE THE SELECTIVE DELIVERY OF CYTOTOXIC AGENTS THROUGH THE USE OF BISPECIFIC ANTIBODIES. TAXOL IS A PROMISING NEW ANTICANCER AGENT ISOLATED IN LOW YIELD FROM THE BARK OF THE PACIFIC YEW TREE, TAXUS BREVIFOLIA. THE APPLICANT WILL PREPARE A THIOETHER-LINKED BISPECIFIC ANTIBODY WITH DIAL SPECIFICITY FOR TAXOL AND HUMAN CANCER CELLS. IT IS ASSERTED THAT THIS BISPECIFIC ANTIBODY WILL POTENT ...

HIGH AFFINITY ANTIGEN-ANTIBODY BINDING IS ESSENTIALLY ANHYDROUS HYDROPHOBIC ORGANIC SOLVENTS HAS BEEN DEMONSTRATED IN OUR LABORATORY. USING AN ESTRADIOL-ANTIESTRADIOL SYSTEM, A PROTOTYPE ORGANIC PHASE RADIOIMMUNOASSAY (OPRIA) HAS BEEN DEVELOPED WHICH DETECTS 200 PG/ML OR ESTRADIOL IN A HEXANE MEDIUM CONTAINING LESS THAT 0.1% (W/W) WATER. THE RESEARCH WILL RESULT IN THE DEVELOPMENT OF AN OPRIA FOR ...

A PALYTOXIN PRODURG DERIVATIVE WILL BE PREPARED. THIS COMPOUND WILL BE TESTED FOR ITS DIFFERENTIAL CYTOTOXICITY COMPARED TO PALYTOXIN; AND FOR ITS ABILITY TO BE ENZYMATICALLY ACTIVATED.

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