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WE WILL CHARACTERIZE THE SERRATIA MARCESENS CHIB GENE BOTH PHYSICALLY AND IN BIOCONTROL EXPERIMENTS. WE WILL GENETICALLY ENGINEER TOBACCO PLANTS TO EXPRESS HIGH LEVELS OF THE CHIB ENCODED CHITINASE IN LEAF TISSUE, THEN WE WILL EXAMINE WHETHER THESE TRANSFORMED PLANTS ARE RESISTANT TO INFECTION BY ALTERNARIA ALTERNARIA, THE CAUSATIVE AGENT IN TOBACCO LEAF BROWN SPOT DISEASE.

WE PROPOSE TO EXAMINE A METHOD OF GENOTYPE INDEPENDENT GENETIC TRANSFORMATION OF ZEA MAYS (MAIZE). THE DNA DELIVERY WILL BE ACCOMPLISHED USING HIGH-VELOCITY MICROPROJECTILES. THE FIRST STEP IN THE EXPERIMENT WILL BE TO TEST THE VARIOUS TISSUES OF MAIZE AS POTENTIAL TARGETS FOR THE INCORPORATION OF DNA. THE TISSUES CHOSEN WILL EACH BE CAPABLE OF TRANSMITTING INTEGRATED DNA TO THE NEXT GENERATION OF ...

GENES CONFERRING BASIC PATHOGENICITY AND HOST RANGE IN A PSEUDOMONAS SYRINGAE PV. SYRINGAE (PSS) STRAIN THAT INFECTS CHERRY, PEACH, APRICOT, PEAR, ALMOND, AND SORGHUM WILL BE LOCALIZED. GENETIC LOCI THAT CONFER THE ABILITY OF PSS TO GROW EPIPHYTICALLY WILL BE IDENTIFIED. THESE GENES, ONCE BETTER DEFINED, WILL BE USED TO CONSTRUCT NON-PATHOGENIC FORMS OF PSS, THAT ORIGINALLY INFECTED PEACH AND ALMO ...

SULFA DRUGS AS A GROUP OF ANTIBACTERIAL AGENTS ARE USED EXTENSIVELY AS MEDICINE AND GROWTH PROMOTANTS IN THE SWINE INDUSTRY, AND THUS POSE A GREAT DANGER TO THE CONSUMERS AS MEAT RESIDUE. THE LACK OF AN UNIQUE ANALYTICAL METHOD, WHICH IS BOTH SENSITIVE AND EASY TO USE, MAKES THEIR ASSAY DIFFICULT AND PROHIBITIVELY COSTLY. IN THIS RESEARCH PROJECT, WE PLAN TO ELIMINATE THIS OBSTACLE BY DEVELOPING A ...

REAL-TIME MEASUREMENT OF MOISTURE IN WOOD PRODUCTS IS OF MAJOR IMPORTANCE IN THE MANUFACTURE OF VALUE-ADDEDED WOOD PRODUCTS SUCH AS PLYWOOD, PARTICLE/FIBER/FLAKEBOARD,ETC. IT IS ALSO IMPORTANT FOR DETERMINING THE VALUE OF RAW MATER IALS SUCH AS WOOD CHIPS AND FLAKES WHICH ARE USUALLY PUR- CHASED BY WEIGHT AND FOR ESTABLISHING PRE-AND POST-DRYING NEEDS. THIS RESEARCH WILL DEMONSTRATE THE FEASIBILIT ...

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COATED SOMATIC EMBRYOS OR "SOMATIC SEEDS" PROVIDE A METHOD TO DIRECTLY DELIVER PLANT MATERIAL FROM TISSUE CULTURE TO THE GREENHOUSE OR FIELD WITHOUT THE LABORIOUS AND EXPENSIVE STEP OF FORMING TRANSPLANTS. DESICCATED SOMATIC SEEDS WOULD HAVE MANY ADVANTAGES OVER HYDRATED SOMATIC SEEDS SUCH AS EASE OF STORAGE DUE TO THE QUIESCENT STATE OF THE EMBRYO WHILE DEHYDRATED. DESICCATION IS A NATURAL PROCES ...

A SOFTWARE PACKAGE CALLED THE INTEGRATED PEST MANAGEMENT (IPMAC) IS PROPOSED FOR DEVELOPMENT USING AN INNOVATIVE RAP ID PROTOTYPING METHODOLOGY. IPMAC WILL USE EXPERT SYSTEM TECHNIQUES TO ACQUIRE THE KNOWLEDGE OF A WORLD-RENOWNED EXPERT IN CITRUS PEST MANAGEMENT, DR. GLENN CARMAN, AND MAKETHAT AVAILABLE TO CITRUS ORCHARD OPERATORS. IPMAC WILL CON-SIST OF TACTICAL RESPONSE MODULES FOR IDENTIFYING P ...

THE APPLICANT FIRM PROPOSES UNDER PHASE I TO PROVE FEASIBLE A PROPOSED UNIQUE AIRBORNE RETARDANT DELIVERY SYSTEM. THE DESIGN OF THE SYSTEM WILL LIMIT THE EFFECT OF AIRCRAFT AND ATMOSPHERIC DISTUBANCES (EG. AIRCRAFT FORWARD VELOCITY, TURBULENCE, CROSS-WINDS, HUMIDITY, ETC.) TO THE AERIAL DELIVERY OF RETARDANT. FURTHER PROPOSED IS TO ALLOW FOR FLEXIBILITY AND DELIVERY RATES TO BETTER MEET CURRENT MA ...