Award Data

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THE SPECIFIC AIM IS TO DEVELOP HUMAN CELL LINES CONTAINING SPECIFIC, CLONED GENES OF THE PATHOGENIC VIRUS, HIV, THE CAUSATIVE AGENT OF AIDS. EACH CELL LINE WILL CONTAIN ONLY ONE INDIVIDUAL GENE OF HIV, EXPRESSED BY A EUKARYOTIC GENE PROMOTER. THESE CELL LINES WILL PROVIDE A NONINFECTIOUS, POSITIVE CONTROL STANDARD FOR SUCH DIAGNOSTIC MOLECULAR TESTS AS SOUTHERN ANALYSIS, NORTHERN ANALYSIS, IN SITU ...

THE HUMAN RETROVIRUSES THAT HAVE BEEN EXTENSIVELY CHARACTERIZED (HTLV-I, HTLV-II, HIV-1, AND HIV-2) ALL HAVE ATROPISM FOR THE PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC). SEROLOGICAL ASSAYS HAVE BEEN USED QUITE SUCCESSFULLY TO IDENTIFY PERSONS WITH PRIOR EXPOSURE TO THESE VIRUSES, BUT THEY FAIL TO DETERMINE A CURRENT INFECTION. VIRUS ISOLATION ASSAYS REQUIRE PROLONGED CO-CULTIVATION OF PATIENTS' PBM ...

TANOX BIOSYSTEMS, INC., HAS GENERATED AN ANTI-IDIOTYPE (ID) MONOCLONAL ANTIBODY (MAB), AB19-4, TO THE HIV-1-NEUTRALIZING MAB, BAT123, WHICH RECOGNIZES AN IMMUNODOMINANT REGION OF GP120. RESEARCH HAS CHARACTERIZED AB19-4 TO BE A PARATOPE-SPECIFIC ANTI-ID MAB THAT MIMICS THE THREE-DIMENSIONAL STRUCTURE OF NEUTRALIZING EPITOPES OF THE VIRUS. FURTHERMORE, POLYCLONAL RESPONSES OBTAINED FROM RABBITS IMM ...

TRANS-LINGUA COMMUNICATIONS, INC., PLANS TO PRODUCE AND VALIDATE A SUBSTANCE ABUSE PREVENTION PROGRAM USING SOUND PORTRAITS (COLLAGES OF AUDIOTAPE SEGMENTS) FROM INTERVIEWS WITH EX-ABUSERS, SIBLINGS, PARENTS, AND PROFESSIONALS ON THE CULTURAL, SOCIAL, ETHNIC, PSYCHOLOGICAL, AND ECONOMIC INFLUENCES LEADING TO SUBSTANCE ABUSE AND RECOVERY. DESIGNED TO INFORM AND HELP EMPOWER LISTENERS, THE AUDIOTAPE ...

THIS PROJECT WILL DEVELOP A THERMAL PROBE TO NONINVASIVELY MEASURE LOCAL BLOOD PERFUSION IN TISSUE. THE DEVICE WILL CONSIST OF A SINGLE PROBE THAT CAN BE ACTIVELY HEATED OR COOLED AND PUT IN CONTACT WITH THE SKIN. THIS CREATES A TRANSIENT HEAT FLUX, WHICH IS MEASURED AS A FUNCTION OF TIME. TOGETHER WITH A SIMULTANEOUS MEASUREMENT OF THE SURFACE TEMPERATURE, THIS ALLOWS CALCULATION OF THE THERMAL D ...

THE GOAL OF THIS RESEARCH IS TO DEVELOP AND ADAPT VESTEC CORPORATION'S "UNIVERSAL INTERFACE" TO COUPLE CONVENTIONAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TO ELECTROSPRAY MASS SPECTROMETRY. THE INTERFACE, WHICH HAS BEEN SUCCESSFULLY APPLIED TO COUPLING REVERSED-PHASE HPLC AT FLOW RATES AS HIGH AS 1.5 ML/MIN OF AQUEOUS EFFLUENT TO ELECTRON IMPACT MASS SPECTROMETRY, PRODUCES AN AEROSOL OF DRO ...

MOTOR OPERATED GATE VALVE OPERABILITY PROBLEMS AND FAILURES AT NUCLEAR POWER PLANTS ARE A MAJOR SAFETY CONCERN AS STATEDIN NRC IE BULLETIN 85-03 AND GENERIC LETTER 89-10. THIS PROPOSAL IS AIMED AT IMPROVING GATE VALVE PERFORMANCE BY (1) DEVELOPING COMPREHENSIVE PERFORMANCE PREDICTION MODELS FOR USE OVER A RANGE OF OPERATING CONDITIONS, (2) DEFINING LIMITS, BASED ON SPECIFIC DESIGNS, BEYOND WHICH E ...