Award Data

IN VITRO CYTOGENETIC TESTS TO EVALUATE THE GENETIC HAZARDS OF CHEMICALS ARE INCREASING IN IMPORTANCE FOR PREDICTING IN VIVO EFFECTS. IN PARTICULAR, STUDIES HAVE DEMONSTRATED (1) THE RELEVANCE OF CHROMOSOMAL MUTATION TO INHERITANCE IN GERMCELLS; (2) THAT SPECIFIC CHROMOSOMAL DEFECTS ARE ASSOCIATED WITH MOST NEOPLASIAS; AND (3) THAT CHROMOSOMAL REARRANGEMENTMAY PLAY A ROLE IN ONCOGENE ACTIVATION. HO ...

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RECENT ADVANCES IN BIOCHEMISTRY AND IMMUNOLOGY NOW MAKE POSSIBLE NEW APPROACHES FOR DETECTION AND TREATMENT OF INFECTIOUS DISEASES. OUR GROUP IS COMMITTED TO SUCH TECHNOLOGICAL APPLICATIONS, AND THIS PHASE I PROPOSAL IS IN RESPONSE TO THE STATED INTEREST OF THE N.I.A.D.A. IN SUCH INNOVATIVE BIOMEDICAL TECHNOLOGIES. (DHHS OMNIBUS SOLICITATION, 3/83). SPECIFICALLY, THIS APPLICATION DESCRIBES OUR INT ...

AS THE USE OF MARIHUANA HAS CONTINUED TO GROW, THE NEED FOR ACCURATE AND RELIABLE MEANS OF MEASURING CANNABINOID LEVELS HAS BECOME MORE URGENT. THIS PHASE I APPLICATION OUTLINES OUR INTEREST IN DEVELOPING SUCH A CANNABINOID ASSAY WHICH WILL BE RAPID, NON-ISOTOPIC, AND CAPABLE OF BEING PERFORMED IN A "FIELD" SETTING. OUR SPECIFIC GOALS FOR PHASE I WILL BE TO GENERATE MURINE MONOCLONAL ANTIBODIES TO ...

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CURRENT TECHNIQUES FOR THE DIAGNOSIS OF GENETIC DISEASE ARE LIMITED BY AN INABILITY TO DETECT THE MAJORITY OF MUTATIONS AND POLYMORPHISMS IN DNA. DNA MOLECULES THAT DIFFER BY ONE OR A FEW BASE PAIRS CAN MOST EFFICIENTLY BE DISTINGUISHED BYDENATURING GRADIENT GEL ELECTROPHORESIS DUE TO SEQUENCE SPECIFIC DIFFERENCES IN MELTING PROPERTIES OF DNA. HOWEVER,BASE CHANGES CAN BE DETECTED ONLY IN DOMAINS O ...

THE OBJECTIVE OF THE PROJECT IS TO ESTABLISH HUMAN HEMATO- POIETIC PROGENITOR GROWTH FACTOR-DEPENDENT CELL LINES IN CULTURE. TO ACCOMPLISH THIS, RESEARCHERS WILL FIRST DESIGN AND CONSTRUCT RETROVIRAL VECTORS THAT CAN INFECT AND EXPRESSCLONED GENES IN APPROPRIATE CELL TYPES. THESE WOULD CARRY BOTH A SELECTABLE MARKER GENE AND AN ONCOGENE. SECOND, RESEARCHERS WILL ESTABLISH MURINE CELL LINES PRODUCI ...

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BOVINE PANCREATIC RIBONUCLEASE A (RNASE A) IS A MODEL SYSTEMFOR ESTABLISHING RULES THAT GOVERN PROTEIN STABILITY AND FOLDING. BY LEARNING AND APPLYING THESE RULES, MORE ROBUST ENZYMES OF VARIOUS TYPES COULD BE DESIGNED FOR COMMERCIAL APPLICATIONS. SYNTHESIS, CLONING AND EXPRESSION IN BACILLUSSUBTILIS OF A GENE ENCODING RNASE A WILL ALLOW FOR THE ECONOMICAL PRODUCTION OF VARIANT PROTEINS WHICH ARE ...