Award Data

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THIS GRANT WILL SEEK THE DEVELOPMENT OF AN AUTOMATED DNA SYNTHESIZER THAT IS ORDERS OF MAGNITUDE FASTER AND MORE COST EFFECTIVE THAN CURRENT COMMERCIAL DNA SYNTHESIZERS WHILE UTILIZING STANDARD DNA CHEMISTRY. THE GOAL IS TO AUTOMATE THE SOLVENT DELIVERY SYSTEM FOR THE WAFER DNA SYNTHESIS DEVICE AND TO EVENTUALLY AUTOMATE ALL ASPECTS OF THE DEVICE. ONCE COMPLETE AUTOMATION IS ACHIEVED, THE WAFER DN ...

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AQUACULTURE IS THE FASTEST GROWING SECTOR IN OUR AGRICULTURAL ECONOMY. THE UPPER MIDWEST HAS HAD LIMITED SUCCESS WITH THE TRADITIONAL AQUACULTURE FISH. COOLWATER FISH THAT COMMAND A HIGH MARKET PRICE AND ARE NATIVE TO THE AREA, SUCH AS WALLEYE, MAY REPRESENT THE GREATEST OPPORTUNITY FOR SUCCESSFUL FISH FARMING IN THE AREAS. FURTHER RESEARCH AND DEVELOPMENT IS NEEDED ON THE INTENSIVE REARING OF WAL ...

THE CILIARY GANGLION INNERVATES SEVERAL IMPORTANT EYE TISSUES, INCLUDING THE CILIARY MUSCLE AND BODY, AND HAS BEEN SHOWN TO REGULATE AQUEOUS OUTFLOW AND ACCOMMODATION. THE EYE DISORDERS, GLAUCOMA AND PRESBYOPIA, MAY INVOLVE THE CILIARY GANGLION THROUGH ITS REGULATION OF AQUEOUS OUTFLOW AND VISUAL ACCOMMODATION. A SUBSTANCE THAT COULD DIRECTLY AND SPECIFICALLY STIMULATE THE CILIARY GANGLION MAY BE ...

BLEPHAROSPASM AND STRABISMUS ARE CHRONIC, DEBILITATING, OCULAR SPASTIC DISORDERS CAUSED BY UNCONTROLLED OR BALANCED MUSCLE CONTRACTION. THE LONG-TERM OBJECTIVE OF THIS WORK IS TO CREATE A THERAPEUTIC AGENT THAT WILL ABLATE CHOLINERGIC NEURONS RESPONSIBLE FOR THE MUSCLE CONTRACTION IN BLEPHAROSPASM AND STRABISMUS, AND TO TREAT HUMANS WITH THESE CONDITIONS. THE SPECIFIC AIMS INPHASE I ARE TO PRODUCE ...

WE WILL CONSTRUCT CHICKEN KH MOUSE HYBRIDOMA CELL LINES SECRETING CHICKEN MABS TO SELECTED ANTIGENS. A PRIMARY ADVANTAGE IN THE CONSTRUCTION OF THESE HYBRIDOMAS IS THE GENETIC DIFFERENCES BETWEEN CHICKENS AND MAMMALS WHICH INCREASES THE CHANCE OF PRODUCING ANTIBODIES AGAINST HIGHLY CONSERVED REGIONS OF MAMMALIAN PROTEINS SUCH AS PEPTIDE HORMONES AND GROWTH FACTORS. BECAUSE OF THIS GENETIC DIFFEREN ...

THE PREPARATION OF IMMUNOTOXINS COMPOSED OF RICIN A AND IMMUNOGLOBULINS HAS CLASSICALLY BEEN PERFORMED WITH HETEROBIFUNCTIONAL CROSSLINKERS SUCH AS N-SUCCINIMIDYL 3-(2-PYRIDYLDITHIO) PROPIONATE (SPDP). THESE CROSSLINKERS REACT WITH THE EPSILON-AMINO GROUPS OF LYSINE ON THE IGG MOLECULE AND THE SULFHYDRYL GROUP OF RICIN A. A COMMON DRAWBACK WITH THIS APPROACH IS THE LACK OF SPECIFIC LOCATION OF AMI ...

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