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RECENT RESEARCH IN REPRODUCTIVE ENDOCRINOLOGY HAS DEMONSTRATED THE IMPORTANCE OF FOLLICLE STIMULATING HORMONE (FSH) IN THE REGULATION OF GAMETOGENESIS AND GONADAL STEROIDPRODUCTION. IT HAS BEEN SHOWN THAT N-LINKED OLIGOSACCHARIDES, ATTACHED TO ASPARAGINE RESIDUES AT SPECIFIC SITES ALONG THE MOLECULE, ARE ESSENTIAL FOR POST-RECEPTOR TARGET CELL ACTIVATION BUT NOT RECEPTOR BINDING. THESE SUGARS ALSO ...

THE OBJECTIVE OF THE PROPOSED RESEARCH PLAN IS TO DEVELOP METHODS TO RAPIDLY SCREEN, ISOLATE, AND CHARACTERIZE COSMID CLONES FROM HUMAN DNA LIBRARIES. IN PHASE I WE WILL ESTABLISH PROCEDURES FOR USING A ROBOTIC WORKSTATION TO AUTOMATE THE SCREENING OF A COSMID LIBRARY PREPARED FROM A SOMATIC CELL HYBRID THAT CONTAINS HUMAN CHROMOSOME 21. WE WILL ALSO OPTIMIZE THE USE OF AN INSTRUMENT THAT CAN DIRE ...

THE LONG-TERM GOAL IS TO CLONE A SET OF DNA MARKERS SPACED EVERY 5 CENTIMORGANS ALONG THE HUMAN GENOME. TO ACHIEVE THIS GOAL, A NOVEL APPROACH HAS BEEN DEVISED TO GENERATE A SET OF NATURALLY SPACED CHROMOSOMAL SIGNPOSTS. THESE SIGNPOSTS WILL ALLOW RAPID CHROMOSOME ASSIGNMENT AND REGIONAL LOCALIZATION OF UNMAPPED SEQUENCES AND GENES. FUTHERMORE, IT IS BELIEVED THAT THIS STRATEGY WILL FACILITATE ORD ...

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THIS PROPOSED EFFORT WILL ESTABLISH AND DISTRIBUTE A REPOSITORY OF HUMAN DNA. A STANDARD METHODOLOGY WILL BE IMPLEMENTED FOR GENERATING DNA BLOTS FOR SPECIFIC INDIVIDUALS. THESE DNA BLOTS WILL CONTAIN FRAGMENTS OF DNA THAT HAVE BEEN GENERATED BY RESTRICTION ENDONUCLEASE TREATMENT, GEL ELECTROPHORESIS, AND TRANSFERRED TO NYLON MEMBRANES. PHASE I ACTIVITY WILL FOCUS ON GENERATING SETS OF THESE DNA B ...

COMPUTER ANALYSIS OF DNA AND PROTEIN SEQUENCE DATA HAS BECOME ESSENTIAL TO RESEARCH IN MODERN MOLECULAR BIOLOGY. SOFTWARE TOOLS ARE USED FOR THE DESIGN OF EXPERIMENTS, THE ACQUISITION OF DATA, AND FOR ANALYSIS OF SEQUENCE DATA, AS EXEMPLIFIED BY THE UNRAVELING OF THE RELATIONSHIPS BETWEEN NORMAL CELLULAR GENES AND ONCO-GENES. FOR PHASE I, WE WISH TO ADD LARGE DATABASE CAPACITY IN A USEFUL FORM TO ...

RAPID METHODS OF DIAGNOSIS IN SUCH AREAS AS CANCER ETIOLOGY, SUSCEPTIBILITY, AND THE IDENTIFICATION OF GENETICALLY HERITABLE DISEASES CURRENTLY INVOLVE A NUMBER OF TIME-CONSUMING STEPS. ELECTROBLOTTING IS A TECHNIQUE WHEREBY ELECTRIC FIELDS ARE USED TO TRANSFER MACROMOLECULES FROM GELS TO SUPPORT MEMBRANES. THE TECHNIQUE CAN BE USED IN STUDIES INVOLVING DNA (SOUTHERN RECENTLY, A NEW BLOTTING SYSTE ...

THE ABILITY TO GROW, MAINTAIN, AND CONDUCT EXPERIMENTS ON CELL CULTURES DERIVED FROM BIOPSIES AND SURGICAL SPECIMENS FROM HUMAN CANCER PATIENTS WOULD BE OF POTENTIAL BENEFIT TO ONCOLOGISTS IN THEIR SEARCH FOR, AND SELECTION OF, APPROPRIATE THERAPEUTIC MODALITIES USED IN TREATMENT OF THEIR PATIENTS. WE BELIEVE THE USE OF KARYON TECHNOLOGY'S GELTRAP IN VITRO CELL CULTURE SYSTEM WOULD FACILITATE THE ...

PERFLUOROCHEMICALS, DESPITE INSOLUBILITY IN WATER, HAVE SHOWN PROMISE AS OXYGEN CARRIERS IN ACELLULAR BLOOD SUBSTITUTES. THE SURFACTANT USED FOR PERFLUOROCHEMICAL EMULSIONS IS MILDLY TOXIC, AND FLUOSOL-DA 20% EMULSIONS (THE MOST EXTENSIVELY TESTED) ARE STABLE ONLY WHEN FROZEN. OUR RESEARCH OBJECTIVE IS TO DEVELOP SUPERIOR EMULSIONS USING A PATENTED HOMOLOGOUS SERIES OF SEMISYNTHETIC PHOSPHOLIPID C ...