Award Data

MOST SYNTHETIC VASCULAR GRAFTS CURRENTLY USED ARE MADE OF EITHER DACRON OR TEFLON. NEITHER OF THESE MATERIALS HAS PROVIDED THE ANSWER FOR VASCULAR REPLACEMENT OF VESSELS OF LESS THAN 6MM. THE OBJECTIVE OF THIS STUDY IS TO TRANSFER TECHNOLOGY ON VASCULAR PROSTHESES MADE FROM A SEGMENTED POLYETHER POLYURETHANE THAT HAS UNIQUE CHARACTERISTICS THAT ENABLE THESE PROSTHESES TO STRETCH AND PULSATE LIKE N ...

SPECIFIC AIMS: WE HAVE RAISED A POTENT MONOCLONAL ANTIBODY (RBC-A) AGAINST BLOOD GROUP A ANTIGEN. OUR AIMS ARE TO: 1. RAISE ADDITIONAL MONOCLONAL ANTIBODIES TO RED BLOOD CELL ALLOANTIGENS; 2. INSTITUTE THE TECHNOLOGY FOR THE PRODUCTION OF HUMAN 3. COMPLETE THE STUDY ON THE COMMERCIAL VALUE OF OUR MONOCLONAL RBC-A AS AN EFFECTIVE IN VITRO DIAGNOSTIC REAGENT. LONG-TERM OBJECTIVES TO HEALTH RELATEDNE ...

TESTING ITS IMMUNOTHERAPEUTIC POTENTIAL THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO DEVELOP PROCESSES FOR THE PRODUCTION OF B-CELL GROWTH FACTOR (BCGF) AND TO TEST THE POTENTIAL APPLICATIONS OF BCGF FOR THE TREATMENT OF HUMAN IMMUNOPATHOLOGICAL CONDITIONS. BCGF MAY BE USEFUL AS AN IMMUNOTHERAPEUTIC AGENT IN THE TREATMENT OF PRIMARY IMMUNODEFICIENCY DISEASES AND POSSIBLY IN CERTAIN B-CELL LEUKEMI ...

THE OBJECTIVE OF THIS RESEARCH PLAN IS TO ISOLATE A CDNA SEQUENCE ENCODING TUMOR NECROSIS FACTOR (TNF) AND, BY MANIPULATION OF THAT SEQUENCE, TO PRODUCE AND SECRETE THE MATURE TNF PROTEIN FROM THE YEAST SACCHAROMYCES CEREVISIAE. PREVIOUS WORK ON TNF HAS SHOWN IT TO HAVE CYTOSTATIC OR CYTOLYTIC ACTIVITY IN VITRO AGAINST A NUMBER OF TRANSFORMED CELL LINES AND TO HAVE TUMOR NECROTIC ACTIVITY AGAINST ...

LYMPHOTOXIN IS A LYMPHOKINE WHICH HAS POTENT ANTITUMOR ACTIVITY. IT IS A GLYCOPROTEIN WITH A MOLECULAR WEIGHT OF 25,000 DALTONS. DUE TO THE MINUTE AMOUNT PRODUCED BY LYMPHOCYTES, EVALUATION OF ITS PHARMACEUTICAL POTENTIAL REQUIRES THAT IT BE CLONED AND EXPRESSED IN A SUITABLE HOST. IN ORDER TO OBTAIN THE CORRECT GLYCOSYLATION PATTERN, WE PROPOSE TO EXPRESS IT IN MAMMALIAN CELLS. IN PHASE I, WE PLA ...

WE PROMISE TO DEVELOP METHODS FOR THE LARGE-SCALE PRODUCTION OF HUMAN COLONY STIMULATING FACTORS (CSF) FROM NORMAL HUMAN PERIPHERAL BLOOD LYMPHOCYTES. THE FERMENTATION METHODS EMPLOYED WILL BE SIMILAR TO THOSE USED FOR THE PRODUCTION OF NATURAL INTERLEUKIN-2. WE ARE PRODUCING HUMAN IL-2 FOR CLINICAL EVALUATION BY THE BIOLOGICAL RESPONSE MODIFIERS PROGRAM (BRMP). PURIFIED CSFS WILL BE TESTED IN PRE ...

WE PROPOSE TO DEVELOP A METHODOLOGY BY WHICH THE GENES CODING FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS CAN BE RAPIDLY CLONED USING RECOMBINANT DNA TECHNIQUES. THE SYSTEM WHICH WE ARE USING LENDS ITSELF TO HIGH-LEVEL EXPRESSION OF THESE ANTIGENS AS HYBRID PROTEINS IN E. COLI. THIS FACILITATES PRODUCTION OF THESE ANTIGENS FOR IN PHASE I A CDNA LIBRARY WILL BE CONSTRUCTED FROM HUMAN THYROID T ...

WE PROPOSE TO IDENTIFY THE GENES FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS BY THE USE OF RECOMBINANT DNA TECHNIQUES. THE SYSTEM THAT WE ARE USING LENDS ITSELF TO HIGH-LEVEL EXPRESSION OF THESE ANTIGENS AS HYBRID PROTEINS IN E. COLI. THIS APPROACH FACILITATES PRODUCTION OF THESE ANTIGENS FOR THE PURPOSE OF ANTIGEN CHARACTERIZATION, GENERATION OF ANTIBODIES AND DEVELOPMENT OF IMMUNODIAGNOSTIC ...

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