Screening Digestive Diseases with Microcantilever Arrays

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$97,295.00
Award Year:
2001
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
54443
Agency Tracking Number:
1R43DK059696-01
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
114 RIDGEWAY CTR, OAK RIDGE, TN, 37830
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
MICHAELKRIGER
() -
Business Contact:
(865) 483-1113
WHITAKER@ATOM-SCI.COM
Research Institute:
n/a
Abstract
DESCRIPTION (Applicant's Abstract): The proposed research is focused on developing a low-cost diagnostic technology to screen for digestive diseases in a clinical setting. The goal is to incorporate screening for multiple diseases into a single test. Our approach is based upon hybridization of DNA to specific peptide nucleic acid (PNA) probes attached to a microcantilever array. By measuring the bending of individual cantilevers induced by hybridization of DNA, mutant and normal alleles of a gene may be identified. The increased stability of PNA/DNA duplexes, compared to that of DNA/DNA, allows monitoring of multiple mutations with a single device. The Phase I work plan is designed to characterize PNA/DNA hybridization on microcantilever arrays by studying the G-to-A transition associated with the disease-causing Cys282Tyr mutation on the HFE gene. In Phase II, large cantilever arrays will be implemented and a prototype instrument will be built to demonstrate the commercial potential of the technology. PROPOSED COMMERCIAL APPLICATIONS: This project is aimed toward developing a low-cost technique for screening genetic diseases. The technology could be utilized for screening of inherited diseases, including the presence of hereditary digestive disease in infants. The technology could also be used for a wide variety of DNA sequence recognition applications, including screening for infectious pathogens in a hospital or as point-of-care in the physician's office, toxic chemicals or radioisotopes in industry or biowarfare, or in veterinary medicine.

* information listed above is at the time of submission.

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