SNP DETECTION WITH UNLABELED, UNAMPLIFIED TARGET DNA

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43HG002404-01
Agency Tracking Number: HG002404
Amount: $99,427.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ATOM SCIENCES, INC., 114 RIDGEWAY CTR, OAK RIDGE, TN, 37830
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 WANDA WHITE
 (865) 483-1113
 WHITE@ATOM-SCI.COM
Business Contact
 TOM WHITAKER
Phone: (865) 483-1113
Email: WHITAKER@ATOM-SCI.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): The goal of the proposed project is to develop an analytical tool for large-scale detection of single-nucleotide polymorphisms (SNPs) in unlabeled, unamplified genomic or pooled nucleic acid samples. A dual hybridization utilizing a labeled, peptide nucleic acid (PNA, a nucleic acid analog) probe and an immobilized DNA probe will be performed. This innovative approach utilizes a long DNA probe to uniquely select the region of DNA near a specific gene and a short PNA probe that allows SNP identification. This simple method for SNP detection could be used in medical clinics for identifying disease causing mutations, such as human coagulation factor V Leiden, and in laboratories to study allele frequency and construct genetic maps. PROPOSED COMMERCIAL APPLICATIONS: This simple method for SNP detection would gain widespread acceptance in clinics for identifying genetic diseases and in laboratories to study allele frequency and construct genetic maps. Atom Sciences plans to market inexpensive kits that would use this technology.

* Information listed above is at the time of submission. *

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