Specific Diagnostic for Members of Genus Mycobacterium

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43AI064969-01
Agency Tracking Number: AI064969
Amount: $99,998.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solitcitation Year: 2005
Solitcitation Topic Code: N/A
Solitcitation Number: PHS2005-2
Small Business Information
Atom Sciences, Inc., 114 Ridgeway Ctr, Oak Ridge, TN, 37830
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (865) 483-1113
Business Contact
Phone: (865) 483-1113
Research Institution
DESCRIPTION (provided by applicant): A novel microarray-based system is proposed for diagnosis of tuberculosis and tuberculosis-like respiratory infections. This diagnostic system offers a parallel method to screen clinical samples for the presence of DNA sequences from Mycobacterium tuberculosis, the causative agent of human tuberculosis, and closely related mycobacterial species, while simultaneously screening specimens for more distantly related mycobacteria and other common infectious agents known to cause tuberculosis-like symptoms. The system selectively amplifies targeted DNA sequences using repeated rounds of primer-directed extension. Unlike the PCR, this method generates short extension products whose lengths are limited by using one of the building blocks in a form that terminates the extension reaction when it is incorporated. Restricting the extension reaction to the production of short extension products allows screening for many pathogens with a single reaction. The technique is very selective because a signal is produced only when the extension reaction products hybridize to probes immobilized on the microarray. This requires that: 1), the primer binds with a complementary sequence on the targeted DNA, and 2) the resulting short extension sequence is complementary to the probe on the microarray used to detect the extension product. Both of these are very selective processes.

* information listed above is at the time of submission.

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