A NOVEL ELISA FOR THE DETECTION OF ANTIBODIES TO HIV-1

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1992
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
18942
Agency Tracking Number:
18942
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1440 Canal Street, New Orleans, LA, 70112
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Craig Cohen
(504) 523-4226
Business Contact:
() -
Research Institute:
n/a
Abstract
THE LONG-TERM OBJECTIVE OF THIS CLINICAL RESEARCH IS TO FURTHER DEVELOP A NOVEL ELISA FOR THE DETECTION OF ANTIBODIES TO HIV-1 ENVELOPE GLYCOPROTEINS. THE UTILIZATION OF NATIVE ENVLOPE HIV-1 GLYCOPROTEINS OFFERS THE POSSIBILITY OF DETECTING ANTIBODIES IN PATIENT ERA TO A BROADER RANGE OF ANTIGENIC DETERMINANTS INCLUDING CONFORMATIONAL EPITOPES. THE ENHANCED SENSITIVITY OF THIS NOVEL ELISA WILL BE EVALUATED FOR POTENTIAL PROGNOSTIC CLINICAL UTILITY IN THE EARLY AND MORE EFFECTIVE TREATMENT OF THE HIV-1 INFECTION. A NOVEL METHOD THAT GREATLY SIMPLIFIES THE PREPARATION OF SOLID-PHASE HIV-1 ENVELOPE GLYCOPROTEINS FOR USE IN THIS ELISA HAS BEEN DEVELOPED. THIS METHOD UTILIZES CONCANAVALIN A ABSORBED TO WELLS OF MICROTITER PLATES TO AFFINITY IMMOBILIZE DETERGENT SOLUBILIZED VIRAL GLYCOPROTEINS RELEASED IN CULTURE FLUIDS OF HIV-1 INFECTED CELL LINES GROWN IN SERUM-FREE MEDIUM. SINCE CONCANAVALIN A HAS A GENERAL SPECIFICITY FOR A VARIETY OF GLYCOPROTEINS CONTAINING MANNOSYL RESIDUES, IT SHOULD BE POSSIBLE TO DEVELOP ELISA'S THAT DETECT GLYCOPROTEIN-SPECIFIC ANTIBOIDES TO A DIVERSITY OF ENVELOPED VIRUSES, INCLUDING OTHER HUMAN AND ANIMAL RETROVIRUSES.

* information listed above is at the time of submission.

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