Sub-Diffraction and Sub-Pixel Microscopic Deconvolution

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$102,294.00
Award Year:
2001
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
54508
Agency Tracking Number:
1R43GM060881-01A2
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
877 25TH ST, WATERVLIET, NY, 12189
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
TIMOTHY HOLMES
() -
Business Contact:
(518) 276-2138
HOLMES@AQI.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The resolving power of the computer- and video-based light microscope is limited by two main factors. (1) It is limited by the optical resolving power of the microscope, which is -0.25 micro meter (2) It is limited by the pixel size. Resolving power of less than 0.2 micro meter usually requires an electron microscope. Three dimensional imaging to resolving powers of less than 0.2 micro meter requires either an intermediate voltage electron microscope or a high voltage electron microscope (HVEM), both of which are not readily available at most institutions. Live imaging is not possible with any electron microscope. We propose a novel blind deconvolution software algorithm that will commercially provide subpixel resolving power of well under 0.2 micro meter for live fluorescence microscope imaging. The software will be affordable to most biology laboratories and will run on the Windows NT/98 platform. It will require no add-on equipment for the PC or the microscope, which is a critical factor for acceptance by customers and original-equipment-manufacturers through whom the software will be sold. Algorithm research and test bed experimentation of the Phase 1 feasibility will involve verification of subdiffraction 3D structure of the centrosome, a fabricated test target and a computer simulation. PROPOSED COMMERCIAL APPLICATION: Applications include 2D and 3D fluorescence microscopy (FM) and transmitted light brightfield microscopy. Live FM will have a large scientific impact, since presently there is no commercial means for resolving power under 0.2 micrometers. Market inquiries by us indicate that on the order of 400 units per year should be sold by us and an aggregate of at least 3 times this number should be sold by inevitable enduring competitors who will develop competitive products after our product is introduced.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government