A Novel Approach to Purifying iPSC Derived Cell Cultures

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM114935-01
Agency Tracking Number: R41GM114935
Amount: $225,000.00
Phase: Phase I
Program: STTR
Awards Year: 2015
Solicitation Year: 2015
Solicitation Topic Code: 200
Solicitation Number: PA14-072
Small Business Information
170 CHESTNUT ST, Albany, NY, 12210-1906
DUNS: 808416700
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 SCOTT TENENBAUM
 (518) 439-6963
 stenenbaum@albany.edu
Business Contact
 EDWARD EVELETH
Phone: (518) 331-1133
Email: ted.eveleth@hocuslocus.com
Research Institution
 STATE UNIVERSITY OF NEW YORK AT ALBANY
 ALBANY, NY, 12222-0001
 Nonprofit college or university
Abstract
DESCRIPTION provided by applicant We are pioneering an RNA based switch technology called structurally interacting RNAs sxRNA which utilizes post transcriptional gene regulation as a reporter for miRNA detection Normally RNA binding proteins RBP associate with a andapos stem loop structure to facilitate translation of an upstream coding region by as much as an order of magnitude It is possible to modify the mRNA to modulate translation of the reporter gene by controlling the binding of RBP This is accomplished by creating a trans acting three way junction with an additional RNA such as a miRNA binds and stabilizes the functional structure by base pairing with the flanking regions of the custom designed stem loop Our goal with this proposal is use this selective expression capability to create homogenous populations of cells in culture focusing specifically on stem cells We plan to create a procedure sold as a kit that would eliminate unwanted cells from a differentiated cell population We will accomplish this by using miRNA expression in the desired cells to turn on translation of an antibiotic gene thereby maintaining the desired cells Success with this product development effort will result in a product that has broad appeal to stem cell researchers both for the benefits of increased homogeneity of stem cell cultures and potentially faster development of stem cell related lines and position us to further enhance the technology for use in CHO cells lines for cGMP cell culturing PUBLIC HEALTH RELEVANCE HocusLocus is developing a novel technology to control the expression of an mRNA This technology can be used express an antibiotic resistance gene only in the presence of cells that express a particular miRNA The product being developed will allow stem cell researchers to ensure the homogeneity and potentially speed up culturing of stem cells or differentiated cells by killing unwanted cells based on their miRNA expression patterns

* Information listed above is at the time of submission. *

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