Fluorescent Microsphere Counting for Flow Measurements

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$555,699.00
Award Year:
1997
Program:
SBIR
Phase:
Phase II
Contract:
1 R43 HL53880-01,
Award Id:
29520
Agency Tracking Number:
29520
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
6307 Tamoshan Drive NW, Olympia, WA, 98502
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Clyde Barlow
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
We will develop low cost and accurate methods for regional flow measurements in tissues. This reseaon the working hypothesis that fluorescent microspheres in tissue digests can be accurately and rapifluorescence flow system. The project will develop instrumentation and evaluate procedures to countdigests. During Phase I a continuous flow fluorescent microsphere counting system will be assembledaccurate counting of microspheres determined. Time profiles of microsphere passage will be evaluatedwith the fluorescence spectrophotometry method. Phase I studies will demonstrate the feasibility ofand provide design parameters for automated systems to be constructed during Phase H for testing inenvironments to evaluate its utility and ease of use. This project will provide researchers with a vthe cost and labor involved in regional flow measurements and improve the attraction of using fluorereplace the older radioactive microsphere methods.

* information listed above is at the time of submission.

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