Intelligent Microtome Instrumentation

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R44HL086267-02
Agency Tracking Number: HL086267
Amount: $747,943.00
Phase: Phase II
Program: SBIR
Awards Year: 2009
Solicitation Year: 2009
Solicitation Topic Code: N/A
Solicitation Number: PHS2009-2
Small Business Information
DUNS: 194542296
HUBZone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (360) 867-6053
Business Contact
Phone: (360) 866-1244
Research Institution
DESCRIPTION (provided by applicant): Fluorescent tagging of cells using dyes, fluorescent proteins, and quantum dots forms the basis for many powerful analytical methods in modern biology. Locating these cells within entire animals is necessary to study cell delivery, differentiation and gene expression. The Phase II SBIR research is developing an intelligent microtome to provide the means to locate cells within an intact organ or organism and to capture thin sections containing target cells. This instrument will serially section whole organs or small mammals, record multiple wavelength fluorescence images from the tissue after removal of each section, analyze changes in fluorescent patterns in real time, and automatically collect thin sections from areas where fluorescently tagged cells are present. The compact instrument will mount on a typical laboratory bench. Instruments will be constructed for testing in university research laboratories studying gene therapy, stem cell behavior, pulmonary dysfunction, and gene transfer by bacterial transfection. A niche analysis of the commercial market for the intelligent microtome was performed during Phase I. The analysis indicated broad interest in this low-cost approach to three- dimensional imaging and the selective automated approach to collection of thin sections. PUBLIC HEALTH RELEVANCE: New biomedical methods are being designed to deliver DNA, RNA, or stem cells to targeted locations within the body to treat disease conditions. The research described in this Phase II SBIR proposal will manufacture intelligent microtomes, based upon prototype development studies conducted in Phase I. These new instruments will be evaluated in university research laboratories for their capability to section and image entire research animals, to locate stem cells or cells in which DNA or RNA is expressed, and to collect those cells in thin sections for further examination. This research will help the answer the questions Which cells within the entire organism are affected by the gene or stem cell therapy? and What is going on at a biochemical level within these cells?

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government