You are here

Multiplexed detection and imaging of protein phosphorylation based on soluble nanopolymers

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 9R44CA200040-02
Agency Tracking Number: R44CA200040
Amount: $897,133.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: 100
Solicitation Number: PA14-071
Solicitation Year: 2014
Award Year: 2015
Award Start Date (Proposal Award Date): 2015-07-01
Award End Date (Contract End Date): 2019-06-30
Small Business Information
West Lafayette, IN 47906-4182
United States
DUNS: 965433258
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (765) 490-6834
Business Contact
Phone: (765) 490-6834
Research Institution

DESCRIPTION provided by applicant With recent technical advances multiple important signaling pathways that may be the causes of human malignancy have continuously been discovered and dissected The vast majority of these signaling pathways involve reversible protein phosphorylation and the information on the location and dynamics of phosphorylation provides important mechanisms on how the signaling networks function and interact While translational research gradually shifts from lab models to clinical samples with the ultimate goal
of identifying cancer biomarkers a simple and reliable phosphorylation assay method is still missing for routine detection of phosphorylation in complex and typically heterogeneous clinical samples Through this NIH SBIR Phase II study we will further develop a novel strategy for phosphorylation assay termed pIMAGO phospho imaging which has recently been introduced and commercialized by us into highly effective products for simple and routine phosphorylation assays This novel design takes advantage of not only the quantum size properties of the soluble nanoparticles but also of the multi functionalized nature of the molecule allowing for highly selective sensitive and simple qualitative and quantitative assessment of protein phosphorylation without the use of either radioactive isotopes or expensive phosphospecific antibodies Due to its size and unique properties it also offers the capability for multiplexed detection of phosphorylation and total protein amount simultaneously We propose to optimize the technology for universal on membrane phosphoprotein detection in routine biological and biomedical research In addition we will develop a set of novel pIMAGO based strategies for multiplexed detection of phosphorylation in antibody microarray and functional reversed phase array formats The new applications will add another dimension to traditional biomedical research and development PUBLIC HEALTH RELEVANCE Protein phosphorylation relates to the onset and development of many cancer types and a highly efficient technology for phosphorylation analysis is critical for
cancer research This NIH SBIR Phase II project will support an effort to develop an innovative phosphorylation analysis technology into commercial products that equip researchers with powerful tools and new directions to combat the devastating disease

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government