MEMBRANE-BASED PROCESS FOR THE REMOVAL OF TOXIC AMMONIUM ION PRODUCED DURING MAMMALIAN-CELL CULTURE

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 7469
Amount: $242,195.00
Phase: Phase II
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Bend Research Inc.
64550 Research Rd, Bend, OR, 97701
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Paul Van Eikeren
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
MAMMALIAN CELLS GROWN IN CULTURE EXCRETE AMMONIUM ION THAT ACCUMULATES IN QUANTITIES THAT MAY LIMIT CELL GROWTH AND REDUCE PRODUCT SYNTHESIS. THE USUAL TACTIC FOR LIMITING THE ACCUMULATION OF AMMONIA IS TO REPLACE THE "SPENT" MEDIUMWITH FRESH MEDIUM. SINCE MEDIUM REPLACEMENT INCREASES THE USE OF EXPENSIVE MEDIUM COMPONENTS, AMMONIUM ACCUMULATION LOWERS CELL AND PRODUCT YIELD ON MEDIUM AND INCREASES PRODUCTION COSTS. AN ATTRACTIVE STRATEGY FOR NULLIFYING THE PROBLEMS ASSOCIATED WITH AMMONIUM-ION EXCRETION IS TO PROVIDE A CONTINUOUS PROCESS FOR REMOVING AMMONIUM ION AND MAINTAINING IT AT A SUBINHIBITORY, STEADY-STATE CONCENTRATION. IN THIS PROPOSAL, WE DESCRIBE A MEMBRANE-BASED PROCESS FOR THE SELECTIVE AND CONTINUOUS REMOVAL OF AMMONIUM IONS PRODUCED DURING MAMMALIAN-CELL CULTURE. THE PROCESS PROMISES TO BE EFFECTIVE ON THE BASIS OF PRELIMINARY TECHNICAL AND ECONOMIC ANALYSES.

* information listed above is at the time of submission.

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