CARRIERS

Award Information
Agency:
National Science Foundation
Branch:
N/A
Amount:
$35,000.00
Award Year:
1983
Program:
SBIR
Phase:
Phase I
Contract:
N/A
Agency Tracking Number:
688
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Biochem Technology Inc
66 Great Valley Parkway, Malvern, PA, 19355
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 DANE W. ZABRISKIE
 PRINCIPAL INVESTIGATOR
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE IMPORTANCE OF TISSUE CULTURE IS GROWING AS NEW PRODUCTS DERIVED FROM MAMMALIAN CELLS ARE BEING INTRODUCED. THE LIMITATIONS OF EXISTING EQUIPMENT AND PROCESS TECHNOLOGY FOR THE PRODUCTION OF PRODUCTS DERIVED FROM TISSUE CULTURE IS WELL RECOGNIZED BY THE INDUSTRY. ALTHOUGH NEW APPROACHESARE BEING DEVELOPED, A PROBLEM WHICH HAS RECEIVED ONLY LIMITED ATTENTION HAS BEEN AERATION AND AGITATION IN LARGE SCALE TISSUE CULTURE. THIS RESEARCH PROJECT ADDRESSES THE PROBLEM OF AERATION AND AGITATION IN TISSUE CULTURE AND INTRODUCES A NEW TECHNOLOGY AS A PROPOSED SOLUTION. CERTAINLIQUID PERFLUORINATED CHEMICALS (PFC) HAVE BEEN IDENTIFIED WHICH ARE IMMISCIBLE IN AQUEOUS MIXTURES, ARE BIOLOGICALLY INERT, AND HAVE HIGH AFFINITIES FOR O(2) AND CO(2) OFTEN REACHING 5-10 TIMES THOSE OF WATER. THIS PROCESS WILL USE A DISPERSION OF PFC IN THE AQUEOUS CULTURE MEDIUM TO FACILITATE THE RECOVERY AND RECYCLING OF PURE PFC AND TO INDUCE BULK MIXING IN THE CULTIVATION REACTOR. THE EXPERIMENTAL OBJECTIVE IS TO EVALUATE THE TECHNICAL FEASIBILITY OF THIS SYSTEM BY GROWING MOUSE L CELLS IN SUSPENSION AND ON MICROCARRIERS. THIS RESEARCH COULD LEAD TO REMOVING ONE OF THE IMPORTANT TECHNICAL OBSTACLES TO GROWING CULTURES AT HIGHER CELL DENSITIES WHILE LOWERING CULTIVATION COSTS.

* information listed above is at the time of submission.

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