USE OF LIQUID CARRIER'S IN TISSUE CULTURE FOR TISSUE CULTUREFOR AERATIC

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$125,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
2755
Agency Tracking Number:
2755
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
66 Great Valley Pkwy, Malvern, PA, 19355
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr John R Forro
Principal Investigator
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LIMITATIONS OF EXISTING EQUIPMENT AND PROCESS TECHNOLOGY FOR THE PRODUCTION OF PRODUCTS DERIVED FROM TISSUE CULTURE IS WELL RECOGNIZED IN THE INDUSTRY. THIS RESEARCH PROJECT IS FOCUSED ON THE PROBLEM OF AERATION IN THE CELL CULTIVATION PROCESS OF GROWING TISSUE CULTURES. THIS PROJECT INTRODUCES A NEW TECHNOLOGY OF USING CERTAIN LIQUID PERFLUORINATED CHEMICALS (PFC) WHICH ARE IMMISCIBLE IN AQUEOUS MIXTURES, BIOLOGICALLY INERT AND HAVE AFFINITIES FOR 02 AND C02 OFTEN REACHING 5-10 TIMES THOSE OFWATER AS A TRANSFER MEDIA. THIS PROCESS WILL USE CELL LINESTHAT GROW IN SUSPENSION CULTURE MEDIA DEVOID OF SERUM. THE DISPERSION OF PFC IN THE AQUEOUS CULTURE MEDIUM WILL FACILITATE THE RECOVERY AND RECYCLING OF PURE PFC. THE PFC WILL BE CIRCULATED THROUGH A DRAFT TUBE REACTOR TO CREATE BULK MIXING. GAS EXCHANGE OCCURS DUE TO THE DISEQUILIBRIUM BETWEEN THE TWO PHASES. WE WILL ATTEMPT TO QUANTIFY THE OXYGEN TRANSPORT RATES REQUIRED TO SUPPORT HIGHCULTURE DENSITIES. THIS RESEARCH COULD REMOVE ONE OF THE TECHNICAL OBSTACLES INGROWING TISSUE CULTURES IN HIGH CELL DENSITIES WHILE LOWERING THE COST OF CELL CULTIVATION. CONSIDERATION WILL BE GIVEN TO USE OF THIS NEW PROCESS IN MICROGRAVITY CONDITIONS TO DETERMINE IF THE UNIQUE ATTRIBUTES OF SPACE USING THIS NEW TECHNOLOGY WILL ALLOW INTER-ATOMIC AND INTER-MOLECULAR FORCES TO ENHANCE THE PROCESS.

* information listed above is at the time of submission.

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