Triple Helix-Based Acoustic Plate Mode DNA Biosensor

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 22261
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1993
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Biode Inc.
2 Oakwood Road, Cape Elizabeth, ME, 04107
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Douglas J. McAllister
 (207) 883-1492
Business Contact
Phone: () -
Research Institution
N/A
Abstract
BIODE will utilize the results of its Phase I research to develop (Phase II) and commercialize (Phase III) a totally new solid state biosensor technology for biological measurements. This technology is based upon the merging of two technologies: Triple Helix Nucleic Acid Probes with Acoustic Plate Mode (APM) micro sensors. Recent advances by BIODE in the design and operation of APM devices have resulted in a DNA hybridization sensor principle with excellent sensitivity (nanograms/milliliter), selectivity and temperature stability when used with a model probe system. BIODE has received further funding from the DOE to optimize the electrical properties of the device. We will also attempt the direct electronic in situ sensing of a diagnostically significant DNA gene sequence, which codes for the abundant late matrix antigen of Cytomegalovirus. A known short-coming of the previous work is the need to denature naturally-occurring dsDNA to single strands before the sensor is able to detect it. The current project addresses the issue of dsDNA detection via triple helix capture. The currently-funded DOE contract will provide BIODE with an extremely sensitive detection element, and the work will provide an improved technique for imparting selectivity to the device.

* information listed above is at the time of submission.

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