A Bio-manufacturing process for fabrication of 3D scaffolds with cell culture der

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43RR028022-01
Agency Tracking Number: RR028022
Amount: $99,902.00
Phase: Phase I
Program: SBIR
Awards Year: 2009
Solitcitation Year: 2009
Solitcitation Topic Code: N/A
Solitcitation Number: PHS2009-2
Small Business Information
3D BIOTEK, LLC
3D BIOTEK, LLC, 675 US HIGHWAY 1, NORTH BRUNSWICK, NJ, 08902
Duns: 808494939
Hubzone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 QING LIU
 (732) 729-6270
 QLIU@3DBIOTEK.COM
Business Contact
 QING LIU
Phone: (732) 729-6270
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): The objective of this research is to develop novel 3D biodegradable porous polymer scaffolds with biomimetic extracellular matrix (ECM) coating for tissue engineering application. This SBIR Phase I proposal is to demonstrate the scaffold manufacturing feasibility by using a proprietary precision extruding deposition technique to fabricate porous 3D tissue scaffold with controlled porosity and internal architecture, and then coated the scaffold with a cell culture derived extracellular matrix (ECM) by using cell culture technology. We hypotheze that this cell culture derived biomimetic ECM coating, after proper decellularization and treatment, can offer similar biological performance as conventional tissue derived ECM in tissue engineering application. Toward this goal, we would like to demonstrate that an ECM coating can be generated onto the surface of porous tissue scaffolds. To achieve this aim, we first apply an enabling precision extruding deposition technique to freeformly fabricate porous 3D polycaprolactone (PCL) scaffolds with various porous structures. We then apply cell culture method to produce two types of ECM coatings using human dermal fibroblasts and human mesenchymal stem cells (under osteogeneic differentiation). We would like to further demonstrate that the decellularized biomimetic ECM coatings derived from two different cell lines (fibroblast and mesenchymal stem cells under osteogeneic differentiation) will affect osteo-differentiation of re-seeded mesenchymal stem cells. More specifically, we would like to demonstrate that the osteo- differentiated mesenchymal stem cells derived ECM coating could be used as a better ECM substrate for re-seeded mesenchymal stem cell in terms of the cell attachment, proliferation and differentiation down to the osteogeneic lineage. PUBLIC HEALTH RELEVANCE The project is aimed at developing novel 3D biodegradable porous polymer scaffolds with biomimetic extracellular matrix (ECM) coating for tissue engineering application. In this SBIR Phase I work, we will demonstrate the scaffold manufacturing feasibility by using a proprietary fabrication technique to produce porous 3D tissue scaffold with controlled porosity and internal architecture, and then use a cell culture technique to generate an extracellular matrix (ECM) as biomimetic coating. We believe that this novel manufacturing process will produce scaffolds with desired both biophysical properties and biological performance.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government