CHEMICAL SYNTHESIS OF 50-500 GRAM QUANTITIES OF OLIGONUCLEOTIDES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$720,453.00
Award Year:
1996
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
19062
Agency Tracking Number:
19062
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
109 School Street, Watertown, MA, 02172
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Alex G Bonner
(617) 926-5450
Business Contact:
() -
Research Institute:
n/a
Abstract
CHEMICAL SYNTHESIS OF 50-500 GRAM QUANTITIES OF OLIGONUCLEOTIDES WHICH CAN BE UTILIZED FOR ANTISENSE-DNA THERAPEUTICS AND DIAGNOSTICS. THE RESEARCH OBJECTIVES ARE TO INVESTIGAGE IMPROVED CHEMISTRIES, TO ASSEMBLE A MANUAL DEVICE WHICH WILL UTILIZE SOLID-PHASE CYANOTHYL PHOSPHORAMIDITE OR PHOSPHONATE CHEMISTRIES AND TO TEST THE SYSTEM IN THE SYNTHESIS OS 15-25 RESIDUE SEQUENCES. THE RESEARCH WILL INVOLVE THE DEVELOPMENT OF (A) A NEW SOLID SUPPORT OR A NEW MEANS OF HANDLING LARGE AMOUNTS OF THE EXISTING SOLID SUPPORTS AND (B) A MEANS TO RECYCLE THE COSTLY DNA SYNTHOS SO THAT THEY CAN BE USED IN LARGE EXCESS TO MAXIMIZE STEPWISE YIELDS. THE CAPABILITY OF HIGH STEPWISE YIELDS IS ROUTINE IN SMALL SCALE, COMMERCIAL INSTRUMENTS AND IS AN IMPORTANT FEATURE FOR LARGER SCALE SYNTHESIS IN ORDER TO SIMPLIFY THE SUBSEQUENT PURIFICATION STEPS. THE POTENTIAL APPLICATIONS OF THE RESEARCH ARE FOR THE RAPID AND ECONOMICAL PREPARATION OF QUANTITIES OF OLIGONUCLEOTIDE THAT ARE NECESSARY FOR CLINICAL TESTING OR FOR EVENTUAL MANUFACTURING OF THERAPEUTIC DRUGS OR DIAGNOSTIC REAGENTS. THESE DEVELOPMENTS WILL BE IMPORTANT ACHIEVEMENTS IN TERMS OF ECONOMIC COMPETITIVENESS. IMPROVED METHODS FOR GRAM AMOUNTS OF CHEMICALLY SYNTHESIZED DNA WILL ACCELERATE DRUG DEVELOPMENT, REDUCE COST AND ENHANCE MEDICAL APPLICATIONS OF BIOTECHNOLOGY PRODUCTS.

* information listed above is at the time of submission.

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