RAPID, ACCURATE IDENTIFICATION OF YEASTS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1990
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
7890
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Biolog Inc.
3447 Investment Blvd Ste 3, Hayward, CA, 94545
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
THE INCIDENCE OF INFECTIONS CAUSED BY YEASTS HAS INCREASED IN RECENT YEARS DUE TO INCREASES IN THE NUMBER OF IMMUNO-COMPROMISED PATIENTS (E.G. AIDS). BECAUSE YEASTS GROW SLOWLY, IT IS DIFFICULT BUT IMPORTANT TO DEVELOP RAPID METHODS FOR THEIR IDENTIFICATION. BIOLOG HOLDS TWO PATENTS ON A NEW REDOX METHOD WHICH IDENTIFIES AN ORGANISM BASED ON PATTERNS OF CARBON SOURCE UTILIZATION. THE METHOD EMPLOYS A TETRAZOLIUM DYE TO COLORIMETRICALLY INDICATE CARBON SOURCE OXIDATIONS. PRINCIPAL ADVANTAGES OF THE METHOD ARE: (1)SPEED--FOR MOST MICROORGANISMS TESTS CAN BE COMPLETED IN UNDER 4 HOURS, (2) SIMPLICITY--THIS IS AN EASY TO INOCULATE AND READ SINGLE COLOR CHEMISTRY, AND (3) ACCURACY--DRAWING FROM THOUSANDS OF POTENTIAL CARBON SOURCESSHOULD ENABLE US TO DEVELOP A TEST PANEL THAT IS FAR MORE ACCURATE THAN ANYTHING PRESENTLY AVAILABLE. THIS PHASE I STUDY WILL DEMONSTRATE THE FEASIBILITY OF DEVELOPING A RAPID AND ACCURATE SPECIATION KIT FOR YEASTS. TWO TASKS ARE INVOLVED: (1) THE DEVELOPMENT OF A SUSPENSION MEDIUM COMPATIBLE WITH THE REDOX CHEMISTRY WHICH IS TAILOREDTO THIS DIVERSE GROUP OF YEASTS, AND (2) A SURVEY OF 380 POTENTIAL TEST SUBSTRATES TO DEMONSTRATE THAT NEW AND USEFUL SPECIATION TESTS CAN BE FOUND BASED ON CARBON SOURCE OXIDATION REACTIONS. THE POTENTIAL MARKET FOR THESE KITS ISLARGE, AND INCLUDES HOSPITAL, PUBLIC HEALTH, AND CLINICAL LABORATORIES.

* information listed above is at the time of submission.

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