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Phenotyping All Genes of S. cerevisiae

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM074543-01A1
Agency Tracking Number: GM074543
Amount: $123,238.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2004
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (510) 785-2564
Business Contact
Phone: (510) 785-2564
Research Institution

DESCRIPTION (provided by applicant): The objective of this project is to further develop, miniaturize, and extend the new Phenotype MicroArray (PM) platform technology (1-3) and then utilize this improved technology to genomically analyze biological pathways and gene function in the model eucaryotic microorganism Saccharomyces cerevisiae. The steps will be 1) development of miniaturized, more efficient, and lower cost second generation technology, 2) characterization of all of S. cerevisiae's known genes, 3) construction of a computerized, database integrating this genomic and phenotypic information, and 4) retrospective evaluation and modifications of PM technology to make it more useful and comprehensive. Phase I will primarily be concerned with step 1 of this process.

A major need in current research is to understand, in depth, the function of large numbers of genes. Multiple, independent approaches are needed. Biolog's new PM technology provides a new approach in which one gene at a time is tested by phenotypic assay under thousands of cellular conditions. Testing of large numbers of genes is accomplished by testing a comprehensive collection of isogenic microbial strains with single gene knockouts or other alterations in an automated high-throughput program. With this approach, we can rapidly and efficiently build up a storehouse of information about gene function that will be extremely valuable to biological researchers.

* Information listed above is at the time of submission. *

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