THE OBJECTIVE OF THIS PROJECT IS TO FORMULATE DEFINED SERUM-FREE MEDIA THAT CAN SUSTAIN THE GROWTH OF PRIMARY AND/OR EARLY PASSAGE RODENT EMBRYO CELLS THAT ARE COMMONLY USED IN STUDIES ON THE MECHANISMS OF CARCINOGENESIS AS WELL AS IN THE DEVELOPMENT OF

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,988.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
3150
Agency Tracking Number:
3150
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1007520 Tyler Place, Ijamsville, MD, 21754
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
P. THOMAS IYPE
PRINCIPAL INVESTIGATOR
(301) 831-8810
Business Contact:
() -
Research Institute:
n/a
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO FORMULATE DEFINED SERUM-FREE MEDIA THAT CAN SUSTAIN THE GROWTH OF PRIMARY AND/OR EARLY PASSAGE RODENT EMBRYO CELLS THAT ARE COMMONLY USED IN STUDIES ON THE MECHANISMS OF CARCINOGENESIS AS WELL AS IN THE DEVELOPMENT OF GENETIC TOXICOLOGY ASSAYS. PHASE I STUDIES INVOLVE: A. SELECTION OF A BASE MEDIUM THAT WOULD GIVE A MAXIMUM PROLIFERATIVE POTENTIAL TO SYRIAN HAMSTER EMBRYO CELLS (DETERMINED FROM GROWTH CURVES AT DIFFERENT CELL DENSITIES AND SERUM CONCENTRATIONS) IN VARIOUS CONVENTIONAL BASAL MEDIA AND COMBINATIONS THEREOF. B. PREPARATION OF AN INTERIM MEDIUM THAT YIELDS MAXIMUM ATTACHMENT OF THESE ANCHORAGE-DEPENDENT CELLS TO PLASTIC SUBSTRATA WITHOUT LOSS OF VIABILITY AND DEVELOPMENT OF A SERUM-FREE PROLIFERATION MEDIUM THAT CAN SUPPORT THE GROWTH OF THE ATTACHED CELLS. THIS WILL BE ACHIEVED BY SELECTING THE ADDITIVES (GROWTH FACTORS, HORMONES, LIPOPROTEINS, ETC.) AFTER ASSAYING FOR THEIR DIRECT AND INDIRECT EFFECTS ON CELL GROWTH. DURING PHASE I, PROTOTYPE OF A SERUM-FREE MEDIUM WILL BE PREPARED AND PARTIALLY CHARACTERIZED WITH RESPECT TO ITS ABILITY TO SUPPORT TRANSFORMATION OF HAMSTER EMBRYO CELLS WITH 7,12-DIMETHYLBENZ(A)ANTHRACENE AS A PRELUDE TO FURTHER DEVELOPMENT. THE OBJECTIVE OF PHASE II WILL BE THE DEVELOPMENT OF MORE SPECIALIZED MEDIA FOR THIS AND OTHER CELL TYPES USED IN IN VITRO TRANSFORMATION STUDIES AND THE EVENTUAL PRODUCTION OF APPROPRIATE SERUM-FREE MEDIA ON A COMMERCIAL BASIS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government