ANTIBODY-MEDIATED DETECTION SYSTEMS FOR ACROLEIN-DNA ADDUCTS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
4786
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Biological Research Faculty &
10075-20 Tyler Place, Ijamsville, MD, 21754
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
P Thomas Iype
Principal Investigator
(301) 831-8810
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LONG-TERM OBJECTIVES OF THIS PROJECT ARE (1) TO ESTABLISH A STABLE SOURCE OF WELL-CHARACTERIZED ANTIBODIES TO DNA ADDUCTS OF CARCINOGEN/MUTAGENS SO THAT THESE IMPORTANT PROBES CAN BE MADE AVAILABLE FOR FUNDAMENTAL RESEARCH INTO THE MECHANISM(S) OF CARCINOGENESIS, AND (2) TOPROVIDE SERVICES/ASSAY KITS FOR MONITORING HUMAN POPULATIONSFOR POSSIBLE EXPOSURE TO SOME ENVIRONMENTAL CARCINOGENS BY SCREENING FOR NEOANTIGENS IN BIOLOGICAL SPECIMENS. SPECIFICAIMS FOR THIS PHASE I EFFORT WILL INCLUDE THE PRODUCTION OF ACROLEIN-DNA ADDUCTS BY TREATING CALF THYMUS DNA IN VITRO WITH VARYING CONCENTRATIONS OF THE CHEMICAL TO OBTAIN DNA SAMPLES CONTAINING DIFFERENT LEVELS OF REACTION. THE PROJECT WILL ALSO INCLUDE UTILIZATION OF A NUMBER OF IMMUNOLOGICAL METHODS THAT WOULD REDUCE THE AMOUNT OF IMMUNOGEN OR INCREASE THE EFFICIENCY OF ANTIBODY PRODUCTION BY THE USE OF RBF/DN MICE WITH A MARKER CHROMOSOME THAT CONTAINS THE GENES FOR BOTH APRT AND IMMUNOGLOBIN HEAVY CHAIN; THE USE OF HL-653 CELLS DEFICIENT IN BOTH HPRT AND APRT AS THE FUSION PARTNER; USE OF IN VITRO IMMUNIZATION WITH OR WITHOUT PROTEIN COUPLING; USE OF INTRASPLENIC IMMUNIZATION, OR USE OF ALTERNATE ADJUVANT SYSTEM.

* information listed above is at the time of submission.

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