THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT EPITHELIAL CELL SYSTEMS THAT WILL DETECT A WIDE SPECTRUM OF ONCOGENES THROUGH DNA TRANSFECTION.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4908
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Biological Research Faculty &
10075-20 Tyler Place, Ijamsville, MD, 21754
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 P THOMAS IYPE
 PRINCIPAL INVESTIGATOR
 (301) 831-8810
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT EPITHELIAL CELL SYSTEMS THAT WILL DETECT A WIDE SPECTRUM OF ONCOGENES THROUGH DNA TRANSFECTION. THREE TYPES OF LIVER CELLS REPRESENTING DIFFERENT STAGES OF THE CARCINOGENIC PROCESS WILL BE USED: A NORMAL DIPLOID, AN IMMORTALIZED ANEUPLOID, AND A MUTANT CELL LINE THAT IS TEMPERATURE SENSITIVE FOR TRANSFORMED PHENOTYPE. SPECIFIC AIMS FOR PHASE I INVOLVE: (1) DETERMINATION OF THE TIME COURSE AND DOSE RESPONSE OF DNA UPTAKE IN ORDER TO STANDARD-IZE THE OPTIMAL CONDITIONS OF UPTAKE. THIS WILL BE ACHIEVEDUSING A SENSITIVE ELISA TECHNIQUE THAT ASSAYS FOR BUDR- LABELLED DNA. (2) DETERMINATION OF TRANSFORMATION FREQUEN- CIES INDUCED BY PLASMID DNAS CONTAINING HUMAN HRAS OR V-MYC GENES. CONCLUSIVE RESULTS ON THE BEST TRANSFECTION CONDITIONS (ENHANCEMENT OF DNA UPTAKE BY CELL SURFACE MANIPULATIONS, AND THE EXPRESSION OF ONCOGENE-MEDIATED PHENOTYPE), WILL BE STANDARDIZED. PHASE I RESULTS WILL PROVIDE A STANDARDIZED PROTOCOL FOR FUTURE DNA TRANSFECTION EXPERIMENTS; THESE ARE PLANNED FOR PHASE II USING OTHER CELLTYPES AND ONCOGENES.

* information listed above is at the time of submission.

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