THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT EPITHELIAL CELL SYSTEMS THAT WILL DETECT A WIDE SPECTRUM OF ONCOGENES THROUGH DNA TRANSFECTION.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4908
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Biological Research Faculty &
10075-20 Tyler Place, Ijamsville, MD, 21754
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
P THOMAS IYPE
PRINCIPAL INVESTIGATOR
(301) 831-8810
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT EPITHELIAL CELL SYSTEMS THAT WILL DETECT A WIDE SPECTRUM OF ONCOGENES THROUGH DNA TRANSFECTION. THREE TYPES OF LIVER CELLS REPRESENTING DIFFERENT STAGES OF THE CARCINOGENIC PROCESS WILL BE USED: A NORMAL DIPLOID, AN IMMORTALIZED ANEUPLOID, AND A MUTANT CELL LINE THAT IS TEMPERATURE SENSITIVE FOR TRANSFORMED PHENOTYPE. SPECIFIC AIMS FOR PHASE I INVOLVE: (1) DETERMINATION OF THE TIME COURSE AND DOSE RESPONSE OF DNA UPTAKE IN ORDER TO STANDARD-IZE THE OPTIMAL CONDITIONS OF UPTAKE. THIS WILL BE ACHIEVEDUSING A SENSITIVE ELISA TECHNIQUE THAT ASSAYS FOR BUDR- LABELLED DNA. (2) DETERMINATION OF TRANSFORMATION FREQUEN- CIES INDUCED BY PLASMID DNAS CONTAINING HUMAN HRAS OR V-MYC GENES. CONCLUSIVE RESULTS ON THE BEST TRANSFECTION CONDITIONS (ENHANCEMENT OF DNA UPTAKE BY CELL SURFACE MANIPULATIONS, AND THE EXPRESSION OF ONCOGENE-MEDIATED PHENOTYPE), WILL BE STANDARDIZED. PHASE I RESULTS WILL PROVIDE A STANDARDIZED PROTOCOL FOR FUTURE DNA TRANSFECTION EXPERIMENTS; THESE ARE PLANNED FOR PHASE II USING OTHER CELLTYPES AND ONCOGENES.

* information listed above is at the time of submission.

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