DIFFERENTIATED CELLS AS TARGETS FOR CARCINOGEN-SCREENING

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1992
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
19221
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Biological Research
10075-20 Tyler Place, Ijamsville, MD, 21754
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
P Thomas Iype
(301) 831-8810
Business Contact:
() -
Research Institution:
n/a
Abstract
THE OVERALL OBJECTIVE IS TO DEVELOP A NEW SYRIAN HAMSTER EMBRYO (SHE) MORPHOLOGICAL TRANSFORMATION ASSAY (MTA) EMPLOYING SPECIFIC CELL TYPES AS TARGETS AND USING DEFINED, SERUM-FREE CULTURE MEDIA. SPECIFIC AIMS FOR PHASE I STUDY INCLUDE: (1) ISOLATION AND CHARACTERIZATION OF SPECIFIC CELL TYPES (CHONDROCYTES, MYCOCYTES, FIBROBLASTS, ENDOTHELIAL CELLS) FROM SHE AS TARGETS FOR THE IN VITRO TRANSFORMATION ASSAY; (2) ADAPTATION OF EACH OF THE SELECTED TARGET CELL TYPES TO GROW IN APPROPRIATE SERUM-FREE GROWTH MEDIA; (3) DEVELOPMENT OF A PROTOTYPE SERUM-FREE MEDIUM THAT ENHANCES THE TRANSFORMED PHENOTYPE. TECHNICAL INNOVATION IN THIS PROJECT LIES IN THE SUBSTITUTION OF DEFINED FACTORS IN PLACE OF SERUM AND AVOIDANCE OF THE USE OF X-IRRADIATED "FEEDER" CELLS THAT MIGHT REPRESENT PREFERRED TARGETS FOR PARTICULAR CARCINOGENS WOULD CONSTITUTE AN IMPORTANT IMPROVEMENT OVER CURRENT MTAS. PROCEDURES AND REAGENTS DEVELOPED WILL BE USEFUL IN SCREENING ENVIRONMENTAL CHEMICALS FOR THEIR CARCINOGENIC POTENTIAL, HENCE COMMERCIALLY VALUABLE.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government