Sensitive Molecular Beacons (MB) Based DNA Microarray

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: DAAD13-02-P-0055
Agency Tracking Number: C021-0236
Amount: $69,700.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2002
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
507 Airport Blvd.,, Suite 107, Morrisville, NC, 27560
DUNS: 031777407
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Xue-Feng Wang
 Director of Technology
 (919) 459-6393
 fwang@bio-machines.com
Business Contact
 Rick Sheridan
Title: Chairman and CEO
Phone: (919) 459-6393
Email: rsheridan@bio-machines.com
Research Institution
N/A
Abstract
"The DNA microarray is a powerful new technology that can be applied to the detection of biological warfare agents. For the detection of multiple agents, tremendous challenges lie ahead for the DNA microarray technology regarding on sensitivity,selectivity, reliability and consistence. The central theme of this proposal is to apply and optimize a newly developed DNA probe: Molecular beacons (MB) to DNA microarray applications. MBs are a new class of oligonucleotides that have extremely highsensitivity and selectivity with single base mismatch identification capability. Unlike current methods, MB based DNA microarray is pre-labeled DNA probes so that users will not need to label sample DNA. In addition, MB based DNA microarray will not beaffected by non-target DNA samples because MB has fluorescent signals only when hybridizations occur. By applying MB based DNA microarray, the current DNA microarray problems can be significantly improved so that PCR-less DNA detection is possible forreal BW situations. The proposed research will capitalize upon all of unique properties for MB and for the first time to assemble MB based DNA arrays. Biomachines will build a prototype MB DNA array system which will be: i) highly sensitive; ii) highlyselective; iii) resistant to interference by materials present in biological samples; and iv) resistant to interference or contamination by non-target DNA or DNA fragments. We believe MB based DNA Microa

* Information listed above is at the time of submission. *

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