ENZYME IMMUNOASSAY FOR T-2 TETRAOL

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$500,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
1947
Agency Tracking Number:
1947
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
9932 W 74th St, Eden Prairie, MN, 55344
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr Peter H Duquette
(612) 941-0080
Business Contact:
() -
Research Institute:
n/a
Abstract
MUCH ATTENTION HAS BEEN FOCUSED UPON THE POSSIBLE USE OF TOXIC BIOLOGICAL AGENTS IN BOTH SOUTHEAST ASIA AND AFGANISTAN. SUCH INCIDENTS INDICATE A NEED FOR IMPROVING THE UNITED STATES CAPABILITY TO ACCURATELY DETECT THESE TOXINS AT LEVELS WELL BELOW THOSE LETHAL TO HUMANS.ONE OF THE MOST COMMON GROUPS OF NATURALLY OCCURING TOXINS IS THE TRICHOTHECENE (E.G., T-2 TOXIN) WHICH ARE HIGHLY TOXIC SECONDARY METABOLITES OF MOLDS THAT HAVE BEEN IDENTIFIED AS THE CAUSE OF TOXICOSESIN HUMANS AND ANIMALS. THE U.S. ARMY HAS SPECULATED THAT T-2 TOXIN HAS BEEN USED AND THUS HAS SOUGHT ASSISTANCE IN DEVELOPING ANALYTICAL METHODS FOR DETECTION OF T-2 TOXIN AND ITS CHIEF URINARY METABOLITE T-2 TETRAOL. A VARIETY OF METHODS ARE AVAILABLE FOR DETERMINING POSSIBLE MYCOTOXIN CONTAMINATION IN BIOLOGICAL FLUIDS OR THE ENVIRONMENT. MOST OF THE AVAILABLE ASSAYS (E.G., HPLC, GC-MS) REQUIRE EXPENSIVE EQUIPMENT, TRAINED PERSONNEL, AND ARE SLOW IN DETERMINING IF CONTAMINATION IS PRESENT. WE PROPOSE TO DEVELOP AN ENZYME IMMUNOASSAY (EIA) WHICH WILL BE USEFUL FOR THE DETECTION OF LOW CONCENTRATIONS OF T-2 TETRAOL IN URINE WHICH IS ALSO ADAPTABLE FOR DETECTION OF OTHER BIOLOGICAL TOXINS. THE ASSAY IS SIMPLE, FAST (LESS THAN 5 MINUTES), NONINSTRUMENTAL AND THUS EASILY PORTABLE FOR FIELD USE BY MILITARY PERSONNEL.

* information listed above is at the time of submission.

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